Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Journal ArticleDOI
A yeast sigma composite element, TY3, has properties of a retrotransposon.
TL;DR: Examination of genomic DNA from five laboratory strains indicates that the 4.7 kilobase pair internal domain is present in one to four copies per haploid genome and that the genomic location of this domain differs from strain to strain.
Journal ArticleDOI
Transposon tagging using Ty elements in yeast.
TL;DR: The ability to induce high levels of Ty transposition is used to develop a method for transposon mutagenesis in Saccharomyces cerevisiae and to construct GAL1-promoted TyH3 or Ty917 elements that contain unique cloning sites, and marked these elements with selectable genes.
Journal ArticleDOI
Induction of yeast Ty element transcription by ultraviolet light
TL;DR: Using differential colony hybridization methods, the cloning of four S. cerevisiae DNA sequences whose transcript levels increase ∼10-fold after a dose of UV irradiation are described, and it is shown that all four sequences possess homology with yeast Ty elements.
Journal ArticleDOI
Amplification and dispersion of repeated DNA sequences in the Triticeae.
TL;DR: Four representatives of a family of dispersed repetitive sequences which were prominent and dispersed in the E genome of Thinopyrum elongatum but poorly represented in wheat, were studied in detail.
Journal ArticleDOI
Characterization of transposable element-associated mutations that alter yeast alcohol dehydrogenase II expression.
TL;DR: Seven cis-dominant, constitutively expressed mutations of the normally glucose-repressible isozyme of alcohol dehydrogenase (ADHII) from the yeast Saccharomyces cerevisiae are caused by insertion of transposable elements from the Ty1 family in front of the ADHII structural gene (ADR2).
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.