Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Integration sites and sequence arrangement of SV40 DNA in a homogeneous series of transformed rat fibroblast lines
TL;DR: Recurrent patterns of hybridizaton were observed in independently isolated rat cell lines, indicating that the sites of the integrative recombinations were close enough, both in the viral and the cellular sequences, not to be distinguished at the level of sensitivity of the technique.
Journal ArticleDOI
Interspersed repetitive DNA sequences are unlikely to be parasitic.
TL;DR: The results indicate that the observed spacing of the sequences is less than that which would maximize recombination, and that no stable equilibrium number of copies of such a gene can be expected according to the simplest assumptions.
Journal ArticleDOI
Innovative Tools and Strategies for Optimizing Yeast Cell Factories.
Grégory Guirimand,Grégory Guirimand,Natalja Kulagina,Nicolas Papon,Tomohisa Hasunuma,Vincent Courdavault +5 more
TL;DR: The most timely and cutting-edge molecular tools and strategies for biosynthetic pathway optimization, combinatorial transcriptional and post-transcriptional engineering, dynamic control of metabolic fluxes, and spatial reconfiguration of metabolic pathways are highlighted.
Journal ArticleDOI
Use of interdelta polymorphisms of Saccharomyces cerevisiae strains to monitor population evolution during wine fermentation.
TL;DR: It is shown that PCR-amplification of DNA delta sequences of S. cerevisiae strains is a reproducible, strain-specific and simple method that can be used successfully to monitor yeast strain population dynamics during wine fermentations.
Journal ArticleDOI
Nonautonomous inverted repeat Alien transposable elements are associated with genes of both monocotyledonous and dicotyledonous plants
TL;DR: PCR on genomic DNA extracts from various monocotyledonous and dicotylingonous plants using the TIR consensus sequence as primer and subsequent hybridization with different Alien probes revealed that these elements are ubiquitously present and highly repeated in the genomes of higher plants.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.