Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Construction of a genetic linkage map in man using restriction fragment length polymorphisms.
TL;DR: A new basis for the construction of a genetic linkage map of the human genome is described, to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA.
Journal ArticleDOI
Somaclonal variation — a novel source of variability from cell cultures for plant improvement
Philip J. Larkin,W. R. Scowcroft +1 more
TL;DR: It is argued that this variation in plant cell culture itself generates genetic variability (somaclonal variation) that may be employed to enhance the exchange required in sexual hybrids for the introgression of desirable alien genes into a crop species.
Journal ArticleDOI
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis
TL;DR: This pulsed field gradient gel electrophoresis fractionates intact S. cerevisiae chromosomal DNA, producing a molecular karyotype that greatly facilitates the assignment of genes to yeast chromosomes.
Journal ArticleDOI
Selfish genes, the phenotype paradigm and genome evolution.
W. F. Doolittle,Carmen Sapienza +1 more
TL;DR: Natural selection operating within genomes will inevitably result in the appearance of DNAs with no phenotypic expression whose only ‘function’ is survival within genomes.
Journal ArticleDOI
The evolutionary dynamics of repetitive DNA in eukaryotes
TL;DR: Features of the organization of repetitive sequences in eukaryotic genomes, and their distribution in natural populations, reflect the evolutionary forces acting on selfish DNA.
References
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Journal ArticleDOI
Controlling elements and the gene.
TL;DR: This paper presented at the 1956 Cold Spring Harbor Symposium provides a brief overview of McClintock's thoughts on controlling elements, including suppressor-mutator, or Spm.
Journal ArticleDOI
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
Marjorie Thomas,Ronald W. Davis +1 more
TL;DR: The five EcoRI† restriction sites in bacteriophage lambda DNA have been mapped and the DNA lengths of the EcoRI fragments are in agreement with their electrophoretic mobility on agarose gels but are not in Agreement with their mobilities on polyacrylamide gels.
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Lambdoid phages that simplify the recovery of in vitro recombinants.
TL;DR: Derivatives of phage λ are described for use as vectors for fragments of DNA generated with the HindIII and EcoRI restriction enzymes to permit the ready distinction between recombinant and vector phages by the colour of the plaques.
Journal ArticleDOI
Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o
TL;DR: One of the eight endonuclease EcoRI fragments of yeast DNA that hybridize to yeast tRNATyr has been identified with the genetically defined nonsense-suppressor locus SUP4 by analyzing the meiotic linkage between the genetic determinant for the SUP4 phenotype and that for an electrophoretic variant of the EcoRI fragment.