Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Journal ArticleDOI
DNA damage activates transcription and transposition of yeast Ty retrotransposons.
TL;DR: Results provide direct evidence that transposable elements can be activated by physical and chemical mutagens/carcinogens and thatTranspositional mutagenesis is induced by these agents in S. cerevisiae.
Journal ArticleDOI
Single base-pair mutations in centromere element III cause aberrant chromosome segregation in Saccharomyces cerevisiae.
TL;DR: It is shown that a 211-base pair segment of CEN3 DNA is sufficient to confer wild-type centromere function in the yeast Saccharomyces cerevisiae, and it is concluded that while some specific nucleotides within the conserved element III sequence are essential for proper centromer function, other conserved nucleotide can be changed.
Journal ArticleDOI
Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.
TL;DR: Results demonstrate that spt mutations can suppress Ty and delta mutations at both HIS4 and LYS2, suggesting that they are general for their effects onTy and delta elements.
Journal ArticleDOI
B104, a new dispersed repeated gene family in Drosophila melanogaster and its analogies with retroviruses.
TL;DR: The sequence data indicate that the B104 element is flanked by a 5 bp direct repeat; that the sequence just internal to the left repeat shares homology with the transfer RNA primer binding site of retroviruses; and thatThe B104 terminal repeat is redundantly transcribed in a manner typical of proviruses of vertebrate retrovirus.
Journal ArticleDOI
Transposon-mediated mutagenesis of a baculovirus
TL;DR: One of the insertions contained a perfect 7-bp inverted terminal repeat, and had caused a direct duplication of 4 bp of viral DNA at the point of insertion, therefore, this insertion sequence has properties of a transposon of the host cell Trichoplusia ni.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.