Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
More filters
Journal ArticleDOI
Retrovirus-like vectors for Saccharomyces cerevisiae: integration of foreign genes controlled by efficient promoters into yeast chromosomal DNA.
TL;DR: Using modified Saccharomyces cerevisiae Ty1 elements located on a 2 mu plasmid, reverse-transcriptase-mediated transposition into yeast chromosomes of expression cassettes containing a foreign gene can be induced.
Journal ArticleDOI
Transitions in topological organization of supercoiled DNA domains as a potential regulatory mechanism
TL;DR: The topological transitions observed in the eukaryotic sequences (relevant in one case for activation of selective in vitro transcription (Ty), in another (ADR2 for the in vivo expression) take place in the range of superhelical densities predicted on theoretical ground.
Journal ArticleDOI
Topological modifications and template activation are induced in chimaeric plasmids by inserted sequences
TL;DR: Observations in chimaeric plasmids suggest a regulatory model of gene expression based on alternative topologies of closed DNA domains, which is not a priori directly related one to the other but appear to be two independent consequences of the same cause: the insertion of foreign DNA sequences into closedDNA domains.
Book ChapterDOI
Genetic Manipulation of Fungi by DNA-Mediated Transformation
P. A. Lemke,M. Peng +1 more
TL;DR: The ability to investigate the genetics of fungi has been extended substantially by DNA-mediated transformation, and few species are suited for laboratory experimentation that includes detailed genetic study.
Journal ArticleDOI
Retrotransposon targeting to RNA polymerase III-transcribed genes
TL;DR: The mechanism by which Ty1 targeting is achieved has been recently solved due to the discovery of an interaction between Ty1 Integrase (IN) and RNA Pol III subunits and the methods used to identify the Ty1-IN interaction with Pol III are described.
References
More filters
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.