Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Partial suppression of an ochre mutation in Saccharomyces cerevisiae by multicopy plasmids containing a normal yeast tRNAGln gene
TL;DR: It is concluded that when expressed in sufficient quantity, normal tRNAGln can weakly suppress the nonsense ochre codon UAA, and suggest that this represents an example of wobble occurring at the first rather than at the third position of the codon.
Journal ArticleDOI
Similarity of the Cin1 repetitive family of Zea mays to eukaryotic transposable elements
Nancy S. Shepherd,Zsuzsanna Schwarz-Sommer,Jutta Blumberg vel Spalve,Manju Gupta,Udo Wienand,Heinz Saedler +5 more
TL;DR: DNA nucleotide sequence data show that some members of the Cin1 middle repetitive family of maize have features characteristic of known transposable elements, including a 6-base pair perfect inverted repeat sequence at its ends.
Journal ArticleDOI
Amplification of rearranged repeated DNA sequences in cereal plants
TL;DR: A simple repeating unit of the rye genome, previously known in tandem arrays, has now been shown to occur also in more complex repeating units, and one such unit has been cloned and found to contain some sequences unrelated to the simple repeat.
Journal ArticleDOI
Structural analysis of templates and RNA polymerase III transcripts of Alu family sequences interspersed among the human β-like globin genes
TL;DR: The data indicate the repetitive DNA sequences, members of the Alu family of interpersed 300 bp reiterated DNA, are imbedded in both templates and the RNAs transcribed from them are composed of an entire AlU family sequence.
Journal ArticleDOI
Discrimination of Saccharomyces cerevisiae wine strains using microsatellite multiplex PCR and band pattern analysis
TL;DR: Simple DNA extraction without the use of phenol, followed by a rapid PCR procedure optimised for multiplex amplification of loci SC8132X, YOR267C and SCPTSY7 and band pattern analysis of the fragments generated by agarose and polyacrylamide gel electrophoresis, has allowed for strain identification among a panel of 30 tested commercial wine strains.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.