Journal ArticleDOI
Expression of a cloned rat brain potassium channel in Xenopus oocytes
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TLDR
Messenger RNA transcribed from RBK-1 in vitro directed the expression of potassium channels when it was injected into Xenopus oocytes and the potassium current through the expressed channels resembles both the transient and delayed rectifier currents reported in mammalian neurons and is sensitive to both 4-aminopyridine and tetraethylammonium.Abstract:
Potassium channels are ubiquitous membrane proteins with essential roles in nervous tissue, but little is known about the relation between their function and their molecular structure. A complementary DNA library was made from rat hippocampus, and a complementary DNA clone (RBK-1) was isolated. The predicted sequence of the 495-amino acid protein is homologous to potassium channel proteins encoded by the Shaker locus of Drosophila and differs by only three amino acids from the expected product of a mouse clone MBK-1. Messenger RNA transcribed from RBK-1 in vitro directed the expression of potassium channels when it was injected into Xenopus oocytes. The potassium current through the expressed channels resembles both the transient (or A) and the delayed rectifier currents reported in mammalian neurons and is sensitive to both 4-aminopyridine and tetraethylammonium.read more
Citations
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Journal ArticleDOI
Biophysical and molecular mechanisms of shaker potassium channel inactivation
TL;DR: A region near the amino terminus with an important role in inactivation has been identified and the results suggest a model where this region forms a cytoplasmic domain that interacts with the open channel to cause inactivation.
Journal ArticleDOI
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs
TL;DR: The subunit stoichiometry of the mammalian K+ channel KV1.1 (RCK1) was examined by linking together the coding sequences of 2-5 K+Channel subunits in a single open reading frame and tagging the expression of individual subunits with a mutation that altered the sensitivity of the channel to block by external tetraethylammonium ion.
Journal ArticleDOI
International Union of Pharmacology. LIII. Nomenclature and Molecular Relationships of Voltage-Gated Potassium Channels
TL;DR: The first cloned potassium channel gene was the Drosophila voltage-gated shaker channel, and this was rapidly followed by the identification of other potassium channel genes.
Journal ArticleDOI
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel
Kevin Ho,Colin G. Nichols,W. J. Lederer,Jonathan Lytton,Peter M. Vassilev,M V Kanazirska,S.C. Hebert +6 more
TL;DR: A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney and the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.
Journal ArticleDOI
Ca2+-activated K+ currents in neurones: types, physiological roles and modulation
TL;DR: Patch-clamp studies have revealed that only one type of AHP can be explained by activation of SKCa channels while a new type of Ca(2+)-activated K+ channel underlies the other, and AHPs in neurones can be divided into two distinct types that are easily separated by kinetic and pharmacological criteria.
References
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
Journal ArticleDOI
A simple and very efficient method for generating cDNA libraries
Ueli Gubler,Beth J. Hoffman +1 more
TL;DR: Using the fully sequenced 1300 nucleotide-long bovine preproenkephalin mRNA, it is established by sequencing that the method yields faithful full-length transcripts.
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Diversity and ubiquity of K channels.
TL;DR: In hippocampal neurons, a Ca-activated K channel which may be responsible for the slow AHP has been identified in single channel recordings, however, this channel has unique properties and it is not blocked by Apamin, suggesting that more than one subclass of Ca- activated K channel may mediate slow AHPs.
Journal ArticleDOI
Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.
Masaharu Noda,Shin Shimizu,Tsutomu Tanabe,Toshiyuki Takai,Toshiaki Kayano,Takayuki Ikeda,Hideo Takahashi,Hitoshi Nakayama,Yuichi Kanaoka,Naoto Minamino,Kenji Kangawa,Hisayuki Matsuo,Michael A. Raftery,Tadaaki Hirose,Seiichi Inayama,Hidenori Hayashida,Takashi Miyata,Shosaku Numa +17 more
TL;DR: Cloning and sequence analysis of cDNA for the Electrophorus electricus electroplax sodium channel indicate that this protein exhibits four repeated homology units, which are presumably oriented in a pseudosymmetric fashion across the membrane.