Gene expression: long-term gene silencing by RNAi.
Nadine L. Vastenhouw,Karin Brunschwig,Kristy L. Okihara,Fritz Müller,Marcel Tijsterman,Ronald H.A. Plasterk +5 more
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TLDR
It is shown that a single episode of RNAi in the nematode Caenorhabditis elegans can induce transcriptional silencing effects that are inherited indefinitely in the absence of the original trigger.Abstract:
Small RNA molecules participate in a variety of activities in the cell: in a process known as RNA interference (RNAi), double-stranded RNA triggers the degradation of messenger RNA that has a matching sequence; the small RNA intermediates of this process can also modify gene expression in the nucleus Here we show that a single episode of RNAi in the nematode Caenorhabditis elegans can induce transcriptional silencing effects that are inherited indefinitely in the absence of the original trigger Our findings may prove useful in the ongoing development of RNAi to treat diseaseread more
Citations
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Histone methylation: a dynamic mark in health, disease and inheritance
Eric L. Greer,Yang Shi +1 more
TL;DR: This work provides a broad overview of how histone methylation is regulated and leads to biological outcomes and suggests its links to disease and ageing and possibly to transmission of traits across generations are illustrated.
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Transgenerational epigenetic inheritance: prevalence, mechanisms, and implications for the study of heredity and evolution
Eva Jablonka,Gal Raz +1 more
TL;DR: The analysis of data shows that epigenetic inheritance is ubiquitous and suggests lines of research that go beyond present approaches to the subject, as well as pointing to the importance of recognizing and understanding epigenetics for practical and theoretical issues in biology.
Journal ArticleDOI
Single-copy insertion of transgenes in Caenorhabditis elegans.
Christian Frøkjær-Jensen,M. Wayne Davis,Chris E. Hopkins,Blake J. Newman,Jason M. Thummel,Søren-Peter Olesen,Morten Grunnet,Erik M. Jorgensen +7 more
TL;DR: A method that inserts a single copy of a transgene into a defined site and verified that single copies are inserted at the targeted site is developed.
Journal ArticleDOI
Strategies for silencing human disease using RNA interference.
Daniel H. Kim,John J. Rossi +1 more
TL;DR: This work has highlighted both promise and challenges in using RNAi for therapeutic applications and suggests design and delivery strategies for RNAi effector molecules must be carefully considered to address safety concerns and to ensure effective, successful treatment of human diseases.
Journal ArticleDOI
piRNAs Can Trigger a Multigenerational Epigenetic Memory in the Germline of C. elegans
Alyson Ashe,Alexandra Sapetschnig,Alexandra Sapetschnig,Eva-Maria Weick,Eva-Maria Weick,Jacinth Mitchell,Marloes P. Bagijn,Marloes P. Bagijn,Amy C. Cording,Amy C. Cording,Anna-Lisa Doebley,Leonard D. Goldstein,Leonard D. Goldstein,Nicolas J. Lehrbach,Nicolas J. Lehrbach,Jérémie Le Pen,Jérémie Le Pen,Greta Pintacuda,Aisa Sakaguchi,Peter Sarkies,Peter Sarkies,Shawn Ahmed,Eric A. Miska,Eric A. Miska +23 more
TL;DR: It is demonstrated that a germline nuclear small RNA/chromatin pathway can maintain stable inheritance for many generations when triggered by a piRNA-dependent foreign RNA response in C. elegans.
References
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Journal ArticleDOI
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
Hiroaki Tabara,Madathia Sarkissian,William G. Kelly,Jamie Fleenor,Alla Grishok,Lisa Timmons,Andrew Fire,Craig C. Mello +7 more
TL;DR: It is shown that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates and the possibility that one natural function of RNAi is transposon silencing is discussed.
Journal ArticleDOI
RNA-mediated non-mendelian inheritance of an epigenetic change in the mouse
Minoo Rassoulzadegan,Minoo Rassoulzadegan,Valérie Grandjean,Valérie Grandjean,Pierre Gounon,Stéphane D. Vincent,Stéphane D. Vincent,Stéphane D. Vincent,Isabelle Gillot,Isabelle Gillot,François Cuzin,François Cuzin +11 more
TL;DR: This work reports a similar modification of the mouse Kit gene in the progeny of heterozygotes with the null mutant Kittm1Alf (a lacZ insertion), identifying an unexpected mode of epigenetic inheritance associated with the zygotic transfer of RNA molecules.
Journal Article
RNAi-mediated pathways in the nucleus
Marjori Matzke,James A. Birchler +1 more
TL;DR: RNA interference is an evolutionarily conserved mechanism that uses short antisense RNAs that are generated by 'dicing' dsRNA precursors to target corresponding mRNAs for cleavage to facilitate the directed silencing of specific genomic regions.
Journal ArticleDOI
RNAi-mediated pathways in the nucleus
Marjori Matzke,James A. Birchler +1 more
TL;DR: RNA interference (RNAi) is an evolutionarily conserved mechanism that uses short antisense RNAs that are generated by 'dicing' dsRNA precursors to target corresponding mRNAs for cleavage as discussed by the authors.
Journal ArticleDOI
A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans
TL;DR: It is shown that C. elegans eri-1 encodes an evolutionarily conserved protein with domains homologous to nucleic-acid-binding and exonuclease proteins that may normally function to limit the duration, cell-type specificity or endogenous functions of RNA interference.
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