Gene Expression Profiling of Tuberculous Meningitis
Ghantasala S. Sameer Kumar,Abhilash K. Venugopal,Lakshmi Dhevi N. Selvan,Arivusudar Marimuthu,Shivakumar Keerthikumar,Swapnali Pathare,Jyoti Bajpai Dikshit,Pramila Tata,Ramesh Hariharan,Thottethodi Subrahmanya Keshava Prasad,H. C. Harsha,Y. L. Ramachandra,Anita Mahadevan,Raghothama Chaerkady,S K Shankar,Akhilesh Pandey +15 more
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TLDR
In this paper, the authors carried out transcriptomic analysis of brain tissue using whole human genome oligonucleotide arrays and identified 2,434 genes that were differentially expressed at least two-fold in TBM cases as compared to controls.Abstract:
Tuberculous meningitis (TBM) is a form of extra pulmonary tuberculosis that is associated with severe neurological deficits and a high mortality. Early diagnosis of TBM is a major challenge despite the availability of several diagnostic methods. Existing diagnostic methods and markers are inadequate for early diagnosis of TBM owing to poor specificity and sensitivity. DNA microarray technology permits high-throughput identification of differentially expressed genes. In order to identify molecules as candidate biomarkers for early diagnosis or as therapeutic targets in TBM, we carried out transcriptomic analysis of brain tissue using whole human genome oligonucleotide arrays. From this gene expression analysis, we identified 2,434 genes that were differentially expressed at least two-fold in TBM cases as compared to controls. The large majority of the differentially expressed genes encoded proteins that are involved in metabolism, cell growth, transport, immune response, cell communication and signal transduction. We confirmed the upregulation of two molecules, serpin peptidase inhibitor, clade A member 3 (SERPINA3) and glial fibrillary acidic protein (GFAP), at the protein level by immunohistochemical analysis. The findings from our study should help us understand the molecular mechanisms underlying TBM and to develop better diagnostic and therapeutic strategies against this deadly disease.read more
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Cerebral tryptophan metabolism and outcome of tuberculous meningitis: an observational cohort study.
Arjan van Laarhoven,Arjan van Laarhoven,Sofiati Dian,Sofiati Dian,Raul Aguirre-Gamboa,Julian Avila-Pacheco,Isis Ricaño-Ponce,Carolien Ruesen,Jessi Annisa,Valerie A. C. M. Koeken,Lidya Chaidir,Lidya Chaidir,Yang Li,Tri Hanggono Achmad,Leo A. B. Joosten,Richard A. Notebaart,Richard A. Notebaart,Rovina Ruslami,Mihai G. Netea,Mihai G. Netea,Marcel M. Verbeek,Bachti Alisjahbana,Vinod Kumar,Clary B. Clish,A Rizal Ganiem,Reinout van Crevel +25 more
TL;DR: Cerebral tryptophan metabolism, which is known to affect Mycobacterium tuberculosis growth and CNS inflammation, is important for the outcome of tuberculous meningitis.
Expression profiling of lymph nodes in tuberculosis patients reveal inflammatory milieu at site of infection
Abhijit Maji,Richa Misra,Anupam Kumar Mondal,Anupam Kumar Mondal,Dhirendra Kumar,Divya Bajaj,Anshika Singhal,Gunjan Arora,Asani Bhaduri,Andaleeb Sajid,Sugandha Bhatia,Sompal Singh,Harshvardhan Singh,Vivek Rao,Debasis Dash,E. Baby Shalini,Joy Sarojini Michael,Anil Chaudhary,Rajesh S. Gokhale,Yogendra Singh +19 more
TL;DR: In this paper, the authors investigated the tissue molecular signature of LNTB patients for the first time and presented findings that indicate the possible mechanism of disease pathology through dysregulation of inflammatory and tissue-repair processes.
Journal ArticleDOI
Expression profiling of lymph nodes in tuberculosis patients reveal inflammatory milieu at site of infection.
Abhijit Maji,Richa Misra,Anupam Kumar Mondal,Anupam Kumar Mondal,Dhirendra Kumar,Divya Bajaj,Anshika Singhal,Gunjan Arora,Asani Bhaduri,Andaleeb Sajid,Sugandha Bhatia,Sompal Singh,Harshvardhan Singh,Vivek Rao,Debasis Dash,E. Baby Shalini,Joy Sarojini Michael,Anil Chaudhary,Rajesh S. Gokhale,Yogendra Singh +19 more
TL;DR: Findings that indicate the possible mechanism of disease pathology through dysregulation of inflammatory and tissue-repair processes are presented, including genes involved in fatty-acid metabolism were found to be downregulated in LNTB suggesting differential lipid metabolic signature.
Journal ArticleDOI
Quantitative proteomics for identifying biomarkers for tuberculous meningitis
Ghantasala S. Sameer Kumar,Abhilash K. Venugopal,Anita Mahadevan,Santosh Renuse,H. C. Harsha,Nandini A. Sahasrabuddhe,Harsh Pawar,Rakesh Sharma,Praveen Kumar,Sudha Rajagopalan,Keith Waddell,Y. L. Ramachandra,Parthasarathy Satishchandra,Raghothama Chaerkady,T. S. Keshava Prasad,K Shankar,Akhilesh Pandey +16 more
TL;DR: This study used a quantitative proteomics approach to discover protein biomarkers for tuberculous meningitis and identified both known and novel differentially regulated molecules, including amphiphysin (AMPH) and neurofascin (NFASC) while ferritin light chain was found to be downregulated in TBM.
Journal ArticleDOI
Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response
Vinuth N Puttamallesh,Sreelakshmi K. Sreenivasamurthy,Pradeep Kumar Singh,H. C. Harsha,Anjali Ganjiwale,Shobha Broor,Akhilesh Pandey,Jayasuryan Narayana,T. S. Keshava Prasad +8 more
TL;DR: This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus and provides an insight into the proteins that are involved as host response factors during an infection.
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