Genetic variation drives seasonal onset of hibernation in the 13-lined ground squirrel.
Katharine R. Grabek,Thomas F. Cooke,Thomas F. Cooke,L. Elaine Epperson,Kaitlyn Spees,Gleyce F. Cabral,Gleyce F. Cabral,Shirley Sutton,Dana K. Merriman,Sandra L. Martin,Carlos Bustamante +10 more
- Vol. 2, Iss: 1, pp 478-478
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TLDR
The power of applying genetic mapping to hibernation is highlighted and new insight is presented into genetics driving its onset, as well as high heritability for hibernation onset.Abstract:
Hibernation in sciurid rodents is a dynamic phenotype timed by a circannual clock. When housed in an animal facility, 13-lined ground squirrels exhibit variation in seasonal onset of hibernation, which is not explained by environmental or biological factors. We hypothesized that genetic factors instead drive variation in timing. After increasing genome contiguity, here, we employ a genotype-by-sequencing approach to characterize genetic variation in 153 ground squirrels. Combined with datalogger records (n = 72), we estimate high heritability (61–100%) for hibernation onset. Applying a genome-wide scan with 46,996 variants, we identify 2 loci significantly (p < 7.14 × 10−6), and 12 loci suggestively (p < 2.13 × 10−4), associated with onset. At the most significant locus, whole-genome resequencing reveals a putative causal variant in the promoter of FAM204A. Expression quantitative trait loci (eQTL) analyses further reveal gene associations for 8/14 loci. Our results highlight the power of applying genetic mapping to hibernation and present new insight into genetics driving its onset. Katherine Grabek et al. use genotype-by-sequencing to characterize genetic variation in 153 ground squirrels, finding high heritability for hibernation onset. They find 14 loci associated with hibernation onset, and a putative causal variant in the promoter of FAM204A.read more
Citations
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Cellular, Molecular, and Physiological Adaptations of Hibernation: The Solution to Environmental Challenges.
TL;DR: This work focuses on obligatory mammalian hibernator to identify the unique challenges they face and the adaptations that allow hibernators to overcome them, and discusses metabolic, neuronal, and hormonal cues that regulate hibernation and how they are thought to be coordinated by internal clocks.
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A unifying, eco-physiological framework for animal dormancy
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Proteomics in Non-model Organisms: A New Analytical Frontier.
TL;DR: This work focuses specifically on eukaryotic organisms and discusses steps to generating sequence databases, analyzing proteomic data with or without a database, interpreting results, and future research opportunities.
Journal ArticleDOI
Accelerated Evolution in Distinctive Species Reveals Candidate Elements for Clinically Relevant Traits, Including Mutation and Cancer Resistance.
Elliott Ferris,Lisa M. Abegglen,Lisa M. Abegglen,Joshua D. Schiffman,Joshua D. Schiffman,Christopher Gregg,Christopher Gregg +6 more
TL;DR: This work identifies accelerated regions (ARs) in the elephant, hibernating bat, orca, dolphin, naked mole rat, and thirteen-lined ground squirrel lineages in mammalian conserved regions, uncovering ~33,000 elements that bind hundreds of different regulatory proteins in humans and mice.
Journal ArticleDOI
Elevated ambient temperature accelerates aspects of torpor phenology in an obligate hibernator
TL;DR: The thirteen-lined ground squirrel (Ictidomys tridecemlineatus) is assumed to be an obligate hibernator, commencing and terminating hibernation on a circannual rhythm, regardless of environmental conditions.
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