High-throughput fingerprinting of human pluripotent stem cell fate responses and lineage bias.
Emanuel J. P. Nazareth,Joel Ostblom,Joel Ostblom,Petra B. Lücker,Shreya Shukla,Manuel Alvarez,Steve Oh,Ting Yin,Peter W. Zandstra +8 more
TLDR
A high-throughput platform to screen hPSCs in configurable microenvironments in which it is demonstrated that short-term responses predict definitive endoderm induction efficiency and can be used to rescue differentiation of cell lines reticent to cardiac induction.Abstract:
This paper describes a platform for high-throughput image-based profiling of human pluripotent stem cells (hPSCs). It is used to quantify differentiation bias and endogenous signaling levels in hPSC lines.read more
Citations
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Regenerative medicine: Current therapies and future directions.
Angelo S. Mao,David J. Mooney +1 more
TL;DR: Developments in fabricating sophisticated grafts and tissue mimics and technologies for integrating grafts with host vasculature will be discussed, and directions for current and future regenerative medicine therapies are proposed.
Journal ArticleDOI
Quality cell therapy manufacturing by design
TL;DR: It is argued here that a solution to this challenge is to design cell manufacturing processes according to quality-by-design (QbD) principles, which integrates scientific knowledge and risk analysis into manufacturing process development and is already being adopted by the biopharmaceutical industry.
Journal ArticleDOI
In vitro cardiac tissue models: Current status and future prospects
TL;DR: This review describes the advances made in area of in vitro cardiac models using biomaterials and bioinspired platforms and envision its applications in the areas of drug screening, disease modeling, and precision medicine.
Journal ArticleDOI
A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction.
Mohsen Afshar Bakooshli,Ethan S. Lippmann,Ben Mulcahy,Nisha Iyer,Christine T Nguyen,Kayee Tung,Bryan A. Stewart,Hubrecht van den Dorpel,Hubrecht van den Dorpel,Tobias Fuehrmann,Molly S. Shoichet,Anne Bigot,Elena Pegoraro,Henry Ahn,Henry Ahn,Howard J. Ginsberg,Howard J. Ginsberg,Mei Zhen,Mei Zhen,Randolph S. Ashton,Penney M. Gilbert +20 more
TL;DR: A three-dimensional co-culture method whereby human muscle progenitors mixed with human pluripotent stem cell-derived motor neurons self-organize to form functional NMJ connections is described, offering a simple method to model and evaluate adult human NMJ de novo development or disease in culture.
Journal ArticleDOI
A stepwise model of reaction-diffusion and positional information governs self-organized human peri-gastrulation-like patterning
Mukul Tewary,Joel Ostblom,Laura Prochazka,Teresa Zulueta-Coarasa,Nika Shakiba,Rodrigo Fernandez-Gonzalez,Peter W. Zandstra +6 more
TL;DR: A high-throughput in vitro system allowing the induction of peri-gastrulation-like fates in geometrically confined hPSC colonies reveals that a two-step process underlies the observed self-organization and subsequent fate acquisition.
References
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Journal ArticleDOI
A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.
TL;DR: A screening window coefficient, called "Z- factor," is defined, which is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment.
Journal ArticleDOI
Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
Stuart M. Chambers,Christopher A. Fasano,Eirini P. Papapetrou,Mark J. Tomishima,Michel Sadelain,Lorenz Studer +5 more
TL;DR: Noggin/SB431542-based neural induction should facilitate the use of hES and hiPS cells in regenerative medicine and disease modeling and obviate the need for protocols based on stromal feeders or embryoid bodies.
Journal ArticleDOI
Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
Noboru Sato,Laurent Meijer,Laurent Meijer,Leandros Skaltsounis,Paul Greengard,Ali H. Brivanlou +5 more
TL;DR: It is shown that activation of the canonical Wnt pathway is sufficient to maintain self-renewal of both HESCs and MESCs, and the use of GSK-3-specific inhibitors such as BIO may have practical applications in regenerative medicine.
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Chemically defined conditions for human iPSC derivation and culture
Guokai Chen,Daniel R. Gulbranson,Daniel R. Gulbranson,Zhonggang Hou,Zhonggang Hou,Jennifer M. Bolin,Victor Ruotti,Mitchell D. Probasco,Kimberly Smuga-Otto,Sara E. Howden,Sara E. Howden,Nicole R. Diol,Nicole R. Diol,Nicholas E. Propson,Nicholas E. Propson,Ryan J. Wagner,Ryan J. Wagner,Garrett O. Lee,Garrett O. Lee,Jessica Antosiewicz-Bourget,Joyce M.C. Teng,James A. Thomson +21 more