Journal ArticleDOI
Impaired Nociception and Pain Sensation in Mice Lacking the Capsaicin Receptor
Michael J. Caterina,A. Leffler,Annika B. Malmberg,William J. Martin,Jodie A. Trafton,K. R. Petersen-Zeitz,Martin Koltzenburg,Allan I. Basbaum,David Julius +8 more
TLDR
Sensory neurons from mice lacking VR1 are severely deficient in their responses to each of these noxious stimuli and are impaired in the detection of painful heat, and showed little thermal hypersensitivity in the setting of inflammation.Abstract:
The capsaicin (vanilloid) receptor VR1 is a cation channel expressed by primary sensory neurons of the "pain" pathway. Heterologously expressed VR1 can be activated by vanilloid compounds, protons, or heat (>43 degrees C), but whether this channel contributes to chemical or thermal sensitivity in vivo is not known. Here, we demonstrate that sensory neurons from mice lacking VR1 are severely deficient in their responses to each of these noxious stimuli. VR1-/- mice showed normal responses to noxious mechanical stimuli but exhibited no vanilloid-evoked pain behavior, were impaired in the detection of painful heat, and showed little thermal hypersensitivity in the setting of inflammation. Thus, VR1 is essential for selective modalities of pain sensation and for tissue injury-induced thermal hyperalgesia.read more
Citations
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Journal ArticleDOI
Functional role of C-terminal cytoplasmic tail of rat vanilloid receptor 1
Viktorie Vlachova,Jan Teisinger,Klara Susankova,A. Lyfenko,Rüdiger Ettrich,Rüdiger Ettrich,Ladislav Vyklický +6 more
TL;DR: Results indicate that the cytoplasmic COOH-terminal domain strongly influences the TRPV1 channel activity, and that the distal half of this structural domain confers specific thermal sensitivity.
Journal ArticleDOI
Intravesical resiniferatoxin for the treatment of interstitial cystitis: a randomized, double-blind, placebo controlled trial.
Christopher K. Payne,Philip G. Mosbaugh,John B. Forrest,Robert J. Evans,Kristene E. Whitmore,Joseph P. Antoci,Ramon Perez-Marrero,Karny Jacoby,Ananias C. Diokno,Keith J. O’Reilly,Tomas L. Griebling,Sandip P. Vasavada,Albert S. Yu,Lyn R. Frumkin +13 more
TL;DR: In the largest prospective, randomized clinical trial reported to date with intravesical vanilloid therapy, single administration of RTX at doses of 0.01 microM to 0.10 microM was not effective in patients with interstitial cystitis.
Journal ArticleDOI
VGLUT2-dependent sensory neurons in the TRPV1 population regulate pain and itch.
Malin C. Lagerström,Katarzyna Rogoz,Bjarke Abrahamsen,Emma Persson,Björn Reinius,Karin Nordenankar,Caroline Ölund,Casey Smith,Jose Alfredo Mendez,Zhou-Feng Chen,John N. Wood,Åsa Wallén-Mackenzie,Klas Kullander +12 more
TL;DR: It is established that V GLUT2 is a major player in TRPV1 thermal nociception and also serves to regulate a normal itch response, demonstrating a dependence on VGLUT2 to maintain normal levels of both histaminergic and nonhistaminergic itch.
Journal ArticleDOI
Pharmacological differences between the human and rat vanilloid receptor 1 (VR1)
Peter McIntyre,Linda M. McLatchie,Anne Chambers,Elsa Phillips,Melanie Clarke,Jonathan Savidge,Christy Toms,Marcus Peacock,Kirti Shah,Janet Winter,Natasha Weerasakera,Michael Webb,Humphrey P. Rang,Stuart Bevan,Iain F. James +14 more
TL;DR: Vanilloid receptors (VR1) were cloned from human and rat dorsal root ganglion libraries and expressed in Xenopus oocytes or Chinese Hamster Ovary cells, finding capsaicin‐induced responses were similar in both cell lines and PPAHV was an agonist at rat VR1 but was virtually inactive at the human VR1.
Journal ArticleDOI
Central and peripheral roles of prostaglandins in pain and their interactions with novel neuropeptides nociceptin and nocistatin
TL;DR: This review focuses on current knowledge concerning mechanisms and pathways for pain induced by prostaglandins and their interactions with novel neuropeptides nociceptin/orphanin FQ and nocistatin derived from the same opioid precursor protein.
References
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Journal ArticleDOI
The capsaicin receptor: a heat-activated ion channel in the pain pathway
Michael J. Caterina,Mark Schumacher,Makoto Tominaga,Tobias A. Rosen,Jon D. Levine,David Julius +5 more
TL;DR: The cloned capsaicin receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.
Journal ArticleDOI
A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia.
TL;DR: Both the thermal method and the Randall‐Selitto mechanical method detected dose‐related hyperalgesia and its blockade by either morphine or indomethacin, but the Thermal method showed greater bioassay sensitivity and allowed for the measurement of other behavioral parameters in addition to the nociceptive threshold.
Journal ArticleDOI
Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide
Peter M. Zygmunt,Jesper Petersson,David Andersson,Huai-hu Chuang,Morten Sørgård,Vincenzo Di Marzo,David Julius,Edward D. Högestätt +7 more
TL;DR: It is shown that the vasodilator response to anandamide in isolated arteries is capsaicin-sensitive and accompanied by release of calcitonin-gene-related peptide (CGRP), which indicates that the vanilloid receptor may be another molecular target for endogenousAnandamide, besides cannabinoid receptors, in the nervous and cardiovascular systems.
Journal Article
Vanilloid (Capsaicin) Receptors and Mechanisms
Arpad Szallasi,Peter M. Blumberg +1 more
TL;DR: This paper focuses on hot pepper, which is eaten on a daily basis by an estimated one-quarter of the world’s population and has potential to be a biological target for regenerative medicine.