Journal ArticleDOI
Impaired Nociception and Pain Sensation in Mice Lacking the Capsaicin Receptor
Michael J. Caterina,A. Leffler,Annika B. Malmberg,William J. Martin,Jodie A. Trafton,K. R. Petersen-Zeitz,Martin Koltzenburg,Allan I. Basbaum,David Julius +8 more
TLDR
Sensory neurons from mice lacking VR1 are severely deficient in their responses to each of these noxious stimuli and are impaired in the detection of painful heat, and showed little thermal hypersensitivity in the setting of inflammation.Abstract:
The capsaicin (vanilloid) receptor VR1 is a cation channel expressed by primary sensory neurons of the "pain" pathway. Heterologously expressed VR1 can be activated by vanilloid compounds, protons, or heat (>43 degrees C), but whether this channel contributes to chemical or thermal sensitivity in vivo is not known. Here, we demonstrate that sensory neurons from mice lacking VR1 are severely deficient in their responses to each of these noxious stimuli. VR1-/- mice showed normal responses to noxious mechanical stimuli but exhibited no vanilloid-evoked pain behavior, were impaired in the detection of painful heat, and showed little thermal hypersensitivity in the setting of inflammation. Thus, VR1 is essential for selective modalities of pain sensation and for tissue injury-induced thermal hyperalgesia.read more
Citations
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Journal ArticleDOI
Oxidative challenges sensitize the capsaicin receptor by covalent cysteine modification.
Huai-hu Chuang,Stephanie C. Lin +1 more
TL;DR: In this article, the capsaicin receptor TRPV1, one of the major transduction channels in the pain pathway, integrates information from extracellular milieu to control excitability of primary nociceptive neurons.
Journal ArticleDOI
Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord.
Long Jun Wu,Long Jun Wu,Bo Duan,Bo Duan,Yi De Mei,Jun Gao,Jun Gao,Jian Guo Chen,Min Zhuo,Lin Xu,Mian Wu,Tian Le Xu,Tian Le Xu +12 more
TL;DR: The idea that the ASICs of dorsal horn neurons participate in central sensory transmission/modulation under physiological conditions and may play important roles in inflammation-related persistent pain is supported.
Journal ArticleDOI
Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons
Sang Hoon Kim,Youngseok Lee,Bradley Akitake,Owen M. Woodward,William B. Guggino,Craig Montell +5 more
TL;DR: It is found that TRPA1 was required in a subset of avoidance GRNs for the behavioral and electrophysiological responses to aristolochic acid, which underscores the evolutionarily conserved role forTRPA1 channels in chemical avoidance.
Journal ArticleDOI
Anandamide regulates neuropeptide release from capsaicin‐sensitive primary sensory neurons by activating both the cannabinoid 1 receptor and the vanilloid receptor 1 in vitro
TL;DR: The data indicate that anandamide may regulate CGRP release from capsaicin‐sensitive primary sensory neurons in vivo, and that the net effect of ananandamide on transmitter release from Capsaicin-sensitivePrimary sensory neurons depends on the concentration of an andamide and the state of the CB1 receptor and VR1.
Journal ArticleDOI
Pharmacological, behavioural and mechanistic analysis of HIV-1 gp120 induced painful neuropathy.
Victoria C.J. Wallace,J. Blackbeard,T. Pheby,Andrew R. Segerdahl,Meirion Davies,F. Hasnie,Sue Hall,Stephen B. McMahon,Andrew S.C. Rice +8 more
TL;DR: Characterised pathological events in the peripheral and central nervous system following application of HIV‐1 gp120 to the rat sciatic nerve suggest that this model is not only useful for the elucidation of mechanisms underlying HIV‐ 1‐related peripheral neuropathy but may prove useful for preclinical assessment of drugs for the treatment of HIV-1 related peripheral neuropathic pain.
References
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Journal ArticleDOI
The capsaicin receptor: a heat-activated ion channel in the pain pathway
Michael J. Caterina,Mark Schumacher,Makoto Tominaga,Tobias A. Rosen,Jon D. Levine,David Julius +5 more
TL;DR: The cloned capsaicin receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.
Journal ArticleDOI
A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia.
TL;DR: Both the thermal method and the Randall‐Selitto mechanical method detected dose‐related hyperalgesia and its blockade by either morphine or indomethacin, but the Thermal method showed greater bioassay sensitivity and allowed for the measurement of other behavioral parameters in addition to the nociceptive threshold.
Journal ArticleDOI
Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide
Peter M. Zygmunt,Jesper Petersson,David Andersson,Huai-hu Chuang,Morten Sørgård,Vincenzo Di Marzo,David Julius,Edward D. Högestätt +7 more
TL;DR: It is shown that the vasodilator response to anandamide in isolated arteries is capsaicin-sensitive and accompanied by release of calcitonin-gene-related peptide (CGRP), which indicates that the vanilloid receptor may be another molecular target for endogenousAnandamide, besides cannabinoid receptors, in the nervous and cardiovascular systems.
Journal Article
Vanilloid (Capsaicin) Receptors and Mechanisms
Arpad Szallasi,Peter M. Blumberg +1 more
TL;DR: This paper focuses on hot pepper, which is eaten on a daily basis by an estimated one-quarter of the world’s population and has potential to be a biological target for regenerative medicine.