Journal ArticleDOI
INCENP loss from an inactive centromere correlates with the loss of sister chromatid cohesion.
TLDR
It is shown for the first time that inactive centromeres are composed of heterochromatin, as defined by the presence of heter Cochromatin protein HP1Hsα, and it is shown that both the inner centromere protein (INCENP) and its binding partner Aurora-B/AIM-1 kinase can also be detected at the inactive Centromere.Abstract:
Inactive centromeres of stable dicentric chromosomes provide a unique opportunity to examine the resolution of sister chromatid cohesion in mitosis. Here we show for the first time that inactive centromeres are composed of heterochromatin, as defined by the presence of heterochromatin protein HP1(Hs alpha). We then show that both the inner centromere protein (INCENP) and its binding partner Aurora-B/AIM-1 kinase can also be detected at the inactive centromere. Thus, targeting of the chromosomal passengers is not dependent upon the presence of an active centromere/kinetochore. Furthermore, we show that the association of INCENP with the inactive centromere correlates strictly with the state of cohesion between sister chromatids: loss of cohesion is accompanied by loss of detectable INCENP. These results are consistent with recent suggestions that one function of the chromosomal passenger proteins may be to regulate sister chromatid separation in mitosis.read more
Citations
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Journal ArticleDOI
Scc1/Rad21/Mcd1 Is Required for Sister Chromatid Cohesion and Kinetochore Function in Vertebrate Cells
Eiichiro Sonoda,Takahiro Matsusaka,Ciaran G. Morrison,Paola Vagnarelli,Osamu Hoshi,Tatsuo Ushiki,Kuniharu Nojima,Tatsuo Fukagawa,Irene C. Waizenegger,Jan-Michael Peters,William C. Earnshaw,Shunichi Takeda +11 more
TL;DR: A role for Scc1 in mitotic regulation, along with cohesion is suggested, although temporal differences exist between eukaryotes in cohesin loss from chromosome arms, as distinct from centromeres.
Journal ArticleDOI
Inactivation of a Human Kinetochore by Specific Targeting of Chromatin Modifiers
Megumi Nakano,Stefano Cardinale,Vladimir N. Noskov,Reto Gassmann,Paola Vagnarelli,Stefanie Kandels-Lewis,Vladimir Larionov,William C. Earnshaw,Hiroshi Masumoto +8 more
TL;DR: The results reveal that a dynamic balance between centromeric chromatin and heterochromatin is essential for vertebrate kinetochore activity.
Journal ArticleDOI
Human centromere repositioning “in progress”
David J. Amor,Karen Bentley,Jacinta Ryan,Jo K. Perry,Lee H. Wong,Howard R. Slater,K. H. Andy Choo +6 more
TL;DR: The stable mitotic and meiotic transmissibility of this pseudodicentric-neocentric chromosome in healthy individuals and the ability of the neocentric activity to form in a euchromatic site in preference to a preexisting alphoid domain provide direct evidence for an inherent mechanism of human centromere repositioning and karyotype evolution "in progress."
Journal ArticleDOI
cis-Acting DNA from Fission Yeast Centromeres Mediates Histone H3 Methylation and Recruitment of Silencing Factors and Cohesin to an Ectopic Site
TL;DR: Recruitment of Rad21-cohesin underscores the link between heterochromatin and chromatid cohesion and indicates that these centromeric elements act independently of kinetochore activity to recruit cohesin.
Journal ArticleDOI
Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I.
María Teresa Parra,Alberto Viera,Rocío Gómez,Jesús Page,Ricardo Benavente,Juan Luis Santos,Julio S. Rufas,José A. Suja +7 more
TL;DR: It is hypothesised that Rad21, and the superimposed SCP3 and SCP2, are involved in the monopolar attachment of sister kinetochores during meiosis I, and are not responsible for the maintenance of sister-chromatid centromere cohesion during meiotic II as previously suggested.
References
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Journal ArticleDOI
Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.
Jer-Yuan Hsu,Zu-Wen Sun,Xiumin Li,Melanie Reuben,Kelly Tatchell,Douglas K. Bishop,Jeremy M. Grushcow,Cynthia J. Brame,Jennifer A. Caldwell,Donald F. Hunt,Rueyling Lin,M. Mitchell Smith,C. David Allis +12 more
TL;DR: An enzyme system that regulates chromosome dynamics and controls histone phosphorylation that is conserved among diverse eukaryotes is revealed.
Journal ArticleDOI
Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase.
TL;DR: It is proposed that in vertebrates, a cleavage-independent pathway removes cohesin from chromosome arms during prophase, whereas a separin-dependent pathway cleaves centromeric cohesIn at the metaphase-anaphase transition.
Journal ArticleDOI
A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite.
TL;DR: DNA binding experiments for proteins immunoprecipitated with anticentromere serum, separated by gel electrophoresis, and transferred to a membrane strongly suggest that the 80-kD antigen specifically binds to the DNA fragment with the 17-bp motif, termed the "CENP-B box."
Journal ArticleDOI
Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation.
TL;DR: It is revealed that INCENP is required for aurora B kinase function and confirmed that the chromosomal passengers have essential roles in mitosis.
Journal ArticleDOI
Splitting the chromosome: cutting the ties that bind sister chromatids.
TL;DR: This review discusses how a number of chromosomal proteins may connect sister chromatids and how they are removed from chromosomes to allow sister Chromatid separation at the onset of anaphase.