Journal ArticleDOI
Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins
TLDR
A previously unrecognized direct signal transduction pathway to the nucleus has been uncovered: IFN-receptor interaction at the cell surface leads to the activation of kinases of the Jak family that phosphorylate substrate proteins called STATs (signal transducers and activators of transcription).Abstract:
Through the study of transcriptional activation in response to interferon alpha (IFN-alpha) and interferon gamma (IFN-gamma), a previously unrecognized direct signal transduction pathway to the nucleus has been uncovered: IFN-receptor interaction at the cell surface leads to the activation of kinases of the Jak family that then phosphorylate substrate proteins called STATs (signal transducers and activators of transcription). The phosphorylated STAT proteins move to the nucleus, bind specific DNA elements, and direct transcription. Recognition of the molecules involved in the IFN-alpha and IFN-gamma pathway has led to discoveries that a number of STAT family members exist and that other polypeptide ligands also use the Jak-STAT molecules in signal transduction.read more
Citations
More filters
Journal ArticleDOI
Signalling pathways in alcohol-induced liver inflammation☆
Pranoti Mandrekar,Gyongyi Szabo +1 more
TL;DR: The key signalling intermediates leading to alcohol-induced inflammation in alcoholic liver disease are described.
Journal ArticleDOI
The emerging safety profile of JAK inhibitors in rheumatic disease
TL;DR: Although nomalignancy signals have been identified to date, long-term follow-up and further research are needed to understand the risk of malignancy associated with these compounds, and vaccination is important to mitigate the risks of these emerging therapies.
Journal ArticleDOI
Silencing of Irf7 pathways in breast cancer cells promotes bone metastasis through immune escape
Bradley N. Bidwell,Bradley N. Bidwell,Clare Y Slaney,Clare Y Slaney,Nimali P. Withana,Nimali P. Withana,Samuel C. Forster,Yuan Cao,Yuan Cao,Sherene Loi,Daniel M. Andrews,Daniel M. Andrews,Thomas Mikeska,Thomas Mikeska,Niamh E. Mangan,Shamith A. Samarajiwa,Nicole Anne De Weerd,Jodee Gould,Pedram Argani,Andreas Möller,Mark J. Smyth,Mark J. Smyth,Robin L. Anderson,Robin L. Anderson,Paul J. Hertzog,Belinda S. Parker,Belinda S. Parker +26 more
TL;DR: An innate immune pathway intrinsic to breast cancer cells is identified, the suppression of which restricts immunosurveillance to enable metastasis and this gene signature may identify patients that could benefit from IFN-based therapies.
Journal ArticleDOI
Immunologic messenger molecules: cytokines, interferons, and chemokines.
TL;DR: For this chapter, cytokines are grouped according to those that are predominantly antigen-presenting cell or T lymphocyte derived; that mediate cytotoxic, humoral, cell mediated, and allergic immunity; or that are immunosuppressive.
Journal ArticleDOI
Identification of a Stat Gene That Functions in Drosophila Development
TL;DR: A Drosophila Stat gene (D-Stat) with a zygotic segmental expression pattern was identified and two D-STAT-binding sites were identified within the eve stripe 3 enhancer region, indicating the JAK-STAT pathway is connected to Drosophile early development.
References
More filters
Journal ArticleDOI
Stimulation of 3T3 cells induces transcription of the c- fos proto-oncogene
TL;DR: Transcription of the c-fos proto-oncogene is greatly increased within minutes of administering purified growth factors to quiescent 3T3 cells, and this stimulation is the most rapid transcriptional response to peptide growth factors yet described, implying a role for c- fos in cell-cycle control.
Journal ArticleDOI
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis
Michael Fried,Donald M. Crothers +1 more
TL;DR: Gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites.
Journal ArticleDOI
Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6
TL;DR: A new family member, Stat3, becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to epidermal growth factor and interleukin-6 but not interferon gamma (IFN-gamma).
Journal ArticleDOI
SH2 and SH3 Domains: Elements that Control Interactions of Cytoplasmic Signaling Proteins
TL;DR: Observations suggest that SH2 and SH3 domains participate in the control of intracellular responses to growth factor stimulation.
Journal ArticleDOI
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system
Mark M. Garner,Arnold Revzin +1 more
TL;DR: It is demonstrated that even when pre-formed in the presence of CAP-cAMP, the polymerase-promoter open complex becomes unstable if CAP is then selectively removed, and this gel method is applied to the study of the E. coli lactose operon regulatory system.
Related Papers (5)
Transcriptional responses to polypeptide ligands: the JAK-STAT pathway.
C Schindler,Jr Je Darnell +1 more