Journal ArticleDOI
Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins
TLDR
A previously unrecognized direct signal transduction pathway to the nucleus has been uncovered: IFN-receptor interaction at the cell surface leads to the activation of kinases of the Jak family that phosphorylate substrate proteins called STATs (signal transducers and activators of transcription).Abstract:
Through the study of transcriptional activation in response to interferon alpha (IFN-alpha) and interferon gamma (IFN-gamma), a previously unrecognized direct signal transduction pathway to the nucleus has been uncovered: IFN-receptor interaction at the cell surface leads to the activation of kinases of the Jak family that then phosphorylate substrate proteins called STATs (signal transducers and activators of transcription). The phosphorylated STAT proteins move to the nucleus, bind specific DNA elements, and direct transcription. Recognition of the molecules involved in the IFN-alpha and IFN-gamma pathway has led to discoveries that a number of STAT family members exist and that other polypeptide ligands also use the Jak-STAT molecules in signal transduction.read more
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Journal ArticleDOI
Activation of p38 MAPK in CD4 T cells controls IL-17 production and autoimmune encephalomyelitis
Rajkumar Noubade,Dimitry N. Krementsov,Roxana del Rio,Tina M. Thornton,Viswas Konasagara Nagaleekar,Naresha Saligrama,Anthony Spitzack,Karen M. Spach,Guadalupe Sabio,Roger J. Davis,Mercedes Rincon,Cory Teuscher +11 more
TL;DR: It is shown that p38 MAPK activation is required for the development and progression of both chronic and relapsing-remitting forms of experimental allergic encephalomyelitis, the principal autoimmune model of multiple sclerosis, and regulation of p38MAPK activity specifically in T cells is sufficient to modulate EAE severity.
Journal ArticleDOI
Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family.
TL;DR: This article showed that Skb1Hs/JBP1, known as a human homolog (Skb 1Hs) of Skb 1 of fission yeast, interacts with NS3 of the hepatitis C virus in a yeast two-hybrid screen.
Book ChapterDOI
HLA class I antigens in human tumors.
TL;DR: This chapter focuses on the abnormal major histocompatibility complex (MHC) expression detected in human tumors as well as on the biological role that these alterations may have in tumor development and the potential therapeutic implications of these discoveries.
Journal ArticleDOI
Activation of endoplasmic reticulum stress response by hepatitis viruses up‐regulates protein phosphatase 2A
TL;DR: The molecular mechanism involved in PP2Ac up‐regulation by HCV and HBV, an important factor leading to an inhibition of IFNα signaling caused by viral protein expression, is described.
Journal ArticleDOI
Transcriptional Induction by Double-stranded RNA Is Mediated by Interferon-stimulated Response Elements without Activation of Interferon-stimulated Gene Factor 3
Sudip K. Bandyopadhyay,George T. Leonard,George T. Leonard,Tanya Bandyopadhyay,George R. Stark,Ganes C. Sen,Ganes C. Sen +6 more
TL;DR: The studies indicate that the major ISRE-mediated signaling pathway used by dsRNA requires interferon regulatory factor 1 and STAT1α, however, this pathway does not require the other known cytoplasmic components used for IFN-α signaling.
References
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Journal ArticleDOI
Stimulation of 3T3 cells induces transcription of the c- fos proto-oncogene
TL;DR: Transcription of the c-fos proto-oncogene is greatly increased within minutes of administering purified growth factors to quiescent 3T3 cells, and this stimulation is the most rapid transcriptional response to peptide growth factors yet described, implying a role for c- fos in cell-cycle control.
Journal ArticleDOI
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis
Michael Fried,Donald M. Crothers +1 more
TL;DR: Gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites.
Journal ArticleDOI
Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6
TL;DR: A new family member, Stat3, becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to epidermal growth factor and interleukin-6 but not interferon gamma (IFN-gamma).
Journal ArticleDOI
SH2 and SH3 Domains: Elements that Control Interactions of Cytoplasmic Signaling Proteins
TL;DR: Observations suggest that SH2 and SH3 domains participate in the control of intracellular responses to growth factor stimulation.
Journal ArticleDOI
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system
Mark M. Garner,Arnold Revzin +1 more
TL;DR: It is demonstrated that even when pre-formed in the presence of CAP-cAMP, the polymerase-promoter open complex becomes unstable if CAP is then selectively removed, and this gel method is applied to the study of the E. coli lactose operon regulatory system.
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