Journal ArticleDOI
Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins
Reads0
Chats0
TLDR
A previously unrecognized direct signal transduction pathway to the nucleus has been uncovered: IFN-receptor interaction at the cell surface leads to the activation of kinases of the Jak family that phosphorylate substrate proteins called STATs (signal transducers and activators of transcription).Abstract:
Through the study of transcriptional activation in response to interferon alpha (IFN-alpha) and interferon gamma (IFN-gamma), a previously unrecognized direct signal transduction pathway to the nucleus has been uncovered: IFN-receptor interaction at the cell surface leads to the activation of kinases of the Jak family that then phosphorylate substrate proteins called STATs (signal transducers and activators of transcription). The phosphorylated STAT proteins move to the nucleus, bind specific DNA elements, and direct transcription. Recognition of the molecules involved in the IFN-alpha and IFN-gamma pathway has led to discoveries that a number of STAT family members exist and that other polypeptide ligands also use the Jak-STAT molecules in signal transduction.read more
Citations
More filters
Journal ArticleDOI
Regulation of Type I Interferon Gene Expression by Interferon Regulatory Factor-3
TL;DR: It is demonstrated that in 293 cells IRF-3 does not stimulate expression of IFN genes but can cooperate with RelA(p65) to stimulate the IFNB promoter.
Journal ArticleDOI
Threading analysis suggests that the obese gene product may be a helical cytokine
TL;DR: A ‘threading’ search of a 3‐dimensional structure database is conducted to determine whether the ob protein might adopt a fold similar to any known structure, and reveals that the ob sequence is compatible with structures from the family of helical cytokines that includes interleukin‐2 and growth hormone.
Journal ArticleDOI
Gab1 Acts as an Adapter Molecule Linking the Cytokine Receptor gp130 to ERK Mitogen-Activated Protein Kinase
Mariko Takahashi-Tezuka,Yuichi Yoshida,Toshiyuki Fukada,Takuya Ohtani,Yojiro Yamanaka,Keigo Nishida,Koichi Nakajima,Masahiko Hibi,Toshio Hirano +8 more
TL;DR: Gab1 was tyrosine phosphorylated in response to various cytokines, such as interleukin-6 (IL-6), IL-3, alpha interferon (IFN-α), and IFN-γ, and that SHP-2, PI-3 kinase, and Ras are involved in Gab1-mediated ERK activation.
Journal ArticleDOI
A Kaposi’s Sarcoma-associated Herpesvirus-encoded Cytokine Homolog (vIL-6) Activates Signaling through the Shared gp130 Receptor Subunit
TL;DR: It is demonstrated that Kaposi’s sarcoma-associated herpesvirus encodes a cytokine that activates specific JAK/STAT signaling via interactions with the gp130 signal transducing subunit independently of the IL-6Rα chain.
Journal ArticleDOI
Isocitrate dehydrogenase mutations suppress STAT1 and CD8+ T cell accumulation in gliomas
Gary Kohanbash,Diego Carrera,Shruti Shrivastav,Brian Ahn,Naznin Jahan,Tali Mazor,Zinal Chheda,Kira Downey,Payal Watchmaker,Casey Beppler,Rolf Warta,Nduka A. Amankulor,Christel Herold-Mende,Joseph F. Costello,Hideho Okada +14 more
TL;DR: The findings demonstrate a mechanism of immune evasion in IDH-MUT gliomas and suggest that specific inhibitors of mutant IDH may improve the efficacy of immunotherapy in patients with IDH -MUTgliomas.
References
More filters
Journal ArticleDOI
Stimulation of 3T3 cells induces transcription of the c- fos proto-oncogene
TL;DR: Transcription of the c-fos proto-oncogene is greatly increased within minutes of administering purified growth factors to quiescent 3T3 cells, and this stimulation is the most rapid transcriptional response to peptide growth factors yet described, implying a role for c- fos in cell-cycle control.
Journal ArticleDOI
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis
Michael Fried,Donald M. Crothers +1 more
TL;DR: Gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites.
Journal ArticleDOI
Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6
TL;DR: A new family member, Stat3, becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to epidermal growth factor and interleukin-6 but not interferon gamma (IFN-gamma).
Journal ArticleDOI
SH2 and SH3 Domains: Elements that Control Interactions of Cytoplasmic Signaling Proteins
TL;DR: Observations suggest that SH2 and SH3 domains participate in the control of intracellular responses to growth factor stimulation.
Journal ArticleDOI
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system
Mark M. Garner,Arnold Revzin +1 more
TL;DR: It is demonstrated that even when pre-formed in the presence of CAP-cAMP, the polymerase-promoter open complex becomes unstable if CAP is then selectively removed, and this gel method is applied to the study of the E. coli lactose operon regulatory system.
Related Papers (5)
Transcriptional responses to polypeptide ligands: the JAK-STAT pathway.
C Schindler,Jr Je Darnell +1 more