Journal ArticleDOI
Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins
Reads0
Chats0
TLDR
A previously unrecognized direct signal transduction pathway to the nucleus has been uncovered: IFN-receptor interaction at the cell surface leads to the activation of kinases of the Jak family that phosphorylate substrate proteins called STATs (signal transducers and activators of transcription).Abstract:
Through the study of transcriptional activation in response to interferon alpha (IFN-alpha) and interferon gamma (IFN-gamma), a previously unrecognized direct signal transduction pathway to the nucleus has been uncovered: IFN-receptor interaction at the cell surface leads to the activation of kinases of the Jak family that then phosphorylate substrate proteins called STATs (signal transducers and activators of transcription). The phosphorylated STAT proteins move to the nucleus, bind specific DNA elements, and direct transcription. Recognition of the molecules involved in the IFN-alpha and IFN-gamma pathway has led to discoveries that a number of STAT family members exist and that other polypeptide ligands also use the Jak-STAT molecules in signal transduction.read more
Citations
More filters
Journal ArticleDOI
Triggering the interferon response: the role of IRF-3 transcription factor.
John Hiscott,Paula M. Pitha,Pierre Génin,Hannah Nguyen,Christophe Heylbroeck,Yael Mamane,Michèle Algarté,Rongtuan Lin +7 more
TL;DR: Recent investigations provide the framework for a model in which virus-dependent phosphorylation of IRF-3 alters protein conformation to permit nuclear translocation, association with transcriptional partners, and primary activation of IFN and IFN-responsive genes.
Journal ArticleDOI
Cloning and expression of a long form of the beta subunit of the interferon alpha beta receptor that is required for signaling.
Paul Domanski,Michael M. Witte,Merril Kellum,Menachem Rubinstein,Rebecca H. Hackett,Paula M. Pitha,Oscar R. Colamonici +6 more
TL;DR: Only expression of the α and long form of the human β subunits in mouse L-929 cells reconstitutes the activation of the Jak kinases and the Stat factors, as well as the antiviral response to human type I IFNs.
Journal ArticleDOI
The Interferon-inducible Ubiquitin-protein Isopeptide Ligase (E3) EFP Also Functions as an ISG15 E3 Ligase
Weiguo Zou,Dong-Er Zhang +1 more
TL;DR: Findings indicate that EFP is an ISG15 E3 ligase for 14-3-3σ in vivo, which suggests a mechanism whereby a limited set of enzymes accomplishes diverse post-translational modifications of their substrates in response to changes in environmental stimulations.
Journal ArticleDOI
Oxidative Stress Triggers STAT3 Tyrosine Phosphorylation and Nuclear Translocation in Human Lymphocytes
Modesto Carballo,Manuel Conde,Rajaa El Bekay,José Martín-Nieto,Marı́a Jesús Camacho,Javier Monteseirín,José Conde,Francisco J. Bedoya,Francisco Sobrino +8 more
TL;DR: It is shown that STAT3 is a transcription factor whose activity is modulated by H2O2 in human lymphocytes, in which endogenous catalase had previously been inhibited, and that H 2O2 and vanadate are likely to inhibit the activity of intracellular tyrosine phosphatase(s), leading to enhanced STAT3 tyosine phosphorylation and hence its translocation to the nucleus.
Journal ArticleDOI
IL-6 cytokine family and signal transduction: a model of the cytokine system
TL;DR: Many functional properties of the IL-6 family of cytokines and their receptors are general features of the cytokine system and its signal transduction mechanisms are described.
References
More filters
Journal ArticleDOI
Stimulation of 3T3 cells induces transcription of the c- fos proto-oncogene
TL;DR: Transcription of the c-fos proto-oncogene is greatly increased within minutes of administering purified growth factors to quiescent 3T3 cells, and this stimulation is the most rapid transcriptional response to peptide growth factors yet described, implying a role for c- fos in cell-cycle control.
Journal ArticleDOI
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis
Michael Fried,Donald M. Crothers +1 more
TL;DR: Gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites.
Journal ArticleDOI
Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6
TL;DR: A new family member, Stat3, becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to epidermal growth factor and interleukin-6 but not interferon gamma (IFN-gamma).
Journal ArticleDOI
SH2 and SH3 Domains: Elements that Control Interactions of Cytoplasmic Signaling Proteins
TL;DR: Observations suggest that SH2 and SH3 domains participate in the control of intracellular responses to growth factor stimulation.
Journal ArticleDOI
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system
Mark M. Garner,Arnold Revzin +1 more
TL;DR: It is demonstrated that even when pre-formed in the presence of CAP-cAMP, the polymerase-promoter open complex becomes unstable if CAP is then selectively removed, and this gel method is applied to the study of the E. coli lactose operon regulatory system.
Related Papers (5)
Transcriptional responses to polypeptide ligands: the JAK-STAT pathway.
C Schindler,Jr Je Darnell +1 more