Loss of mandibular lymph node integrity is associated with an increase in sensitivity to HSV-1 infection in CD118-deficient mice.
TLDR
The adoptive transfer of HSV-specific TCR transgenic CD8+ T cells into CD118−/− mice at the time of infection modestly reduced viral titers in the nervous system suggesting in addition to the generation of HSv-specific CD8 + T cells, other type I IFN-activated pathways are instrumental in controlling acute infection.Abstract:
Type I IFNs are potent antiviral cytokines that contribute to the development of the adaptive immune response. To determine the role of type I IFNs in this process in an infectious disease model, mice deficient in the type I IFN receptor (CD118(-/-)) were ocularly infected with HSV-1 and surveyed at times post infection in the nervous system and lymph node for virus and the host immune response. Virus titers were elevated in the trigeminal ganglia and brain stem with virus disseminating rapidly to the draining lymph node of CD118(-/-) mice. T cell and plasmacytoid dendritic cell infiltration into the brain stem was reduced in CD118(-/-) mice following infection, which correlated with a reduction in CXCL10 but not CXCL9 expression. In contrast, CXCL1 and CCL2 levels were up-regulated in the brainstem of CD118(-/-) mice associated with an increase in F4/80(+) macrophages. By day 5 post infection, there was a significant loss in T, NK, and plasmacytoid dendritic cell numbers in the draining lymph nodes associated with an increase in apoptotic/necrotic T cells and an appreciable lack of HSV-specific CD8(+) T cells. The adoptive transfer of HSV-specific TCR transgenic CD8(+) T cells into CD118(-/-) mice at the time of infection modestly reduced viral titers in the nervous system suggesting in addition to the generation of HSV-specific CD8(+) T cells, other type I IFN-activated pathways are instrumental in controlling acute infection.read more
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CD8+ T Cells Suppress Viral Replication in the Cornea but Contribute to VEGF-C–Induced Lymphatic Vessel Genesis
TL;DR: Results show that CD8+ T cells are required to eliminate virus more efficiently from the cornea but play a minimal role in immunopathology as a source of VEGF-C.
Journal ArticleDOI
APOBEC1-Mediated Editing and Attenuation of Herpes Simplex Virus 1 DNA Indicate That Neurons Have an Antiviral Role during Herpes Simplex Encephalitis
Peter Gee,Yoshinori Ando,Hiroko Kitayama,Seiji P. Yamamoto,Yuka Kanemura,Hirotaka Ebina,Yasushi Kawaguchi,Yoshio Koyanagi +7 more
TL;DR: It is shown that A1 is induced during encephalitis in neurons of rats infected with HSV-1, and sequencing of viral gene UL54 DNA, extracted from infected A1-expressing cells, revealed G- to-A and C-to-T transitions, indicating that A 1 associates with HSv-1 DNA.
Journal ArticleDOI
The Herpes Simplex Virus Type 1 Latency-Associated Transcript Inhibits Phenotypic and Functional Maturation of Dendritic Cells
Aziz Alami Chentoufi,Xavier Dervillez,Gargi Dasgupta,Chelsea Nguyen,Khaled W. Kabbara,Xianzhi Jiang,Anthony B. Nesburn,Steven L. Wechsler,Lbachir BenMohamed +8 more
TL;DR: In this paper, Latency-associated transcript (LAT)-associated T cells were found in latently-infected trigeminal ganglia (TG) of mice infected with LAT, and the effect of LAT on TG resident T cells was investigated.
Journal ArticleDOI
A Functional Type I Interferon Pathway Drives Resistance to Cornea Herpes Simplex Virus Type 1 Infection by Recruitment of Leukocytes.
TL;DR: Mice deficient in the A1 chain of the type I IFN receptor (CD118−/−) are extremely sensitive to ocular infection with low doses of HSV-1 as seen by significantly elevated viral titers in the cornea compared to wild type controls.
Journal ArticleDOI
Loss of the Type I Interferon Pathway Increases Vulnerability of Mice to Genital Herpes Simplex Virus 2 Infection
TL;DR: It is reported that mice deficient in the A1 chain of the type I interferon receptor (CD118−/−) are susceptible to HSV-2 in the absence of medroxyprogesterone preconditioning, and the results underscore the significant contribution of type I IFNs in resistance to genital herpes.
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