Journal ArticleDOI
Metabolic engineering of yeast for production of fuels and chemicals
TLDR
Recent scientific progress in metabolic engineering of S. cerevisiae for the production of bioethanol, advanced biofuels, and chemicals is reviewed.About:
This article is published in Current Opinion in Biotechnology.The article was published on 2013-06-01. It has received 292 citations till now.read more
Citations
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Engineering cytosolic acetyl-coenzyme A supply in Saccharomyces cerevisiae: Pathway stoichiometry, free-energy conservation and redox-cofactor balancing.
TL;DR: It is shown that combination of different acetyl-CoA production pathways may be required to achieve optimal product yields, and underlines that an integral analysis of energy coupling and redox-cofactor balancing in precursor-supply and product-formation pathways is crucial for the design of efficient cell factories.
Journal ArticleDOI
Genome and metabolic engineering in non-conventional yeasts: Current advances and applications.
TL;DR: The challenges of using non-conventional yeasts for strain and pathway engineering are outlined, and the developed solutions to these problems and the resulting applications in industrial biotechnology are discussed.
Journal ArticleDOI
Biotechnological production of aromatic compounds of the extended shikimate pathway from renewable biomass.
TL;DR: Recent strategies for the biotechnological production of aromatic and related compounds from renewable biomass by Escherichia coli, Pseudomonas putida, C. glutamicum and Saccharomyces cerevisiae are summarized and metabolic engineering of the extended shikimate pathway is focused on.
Journal ArticleDOI
Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine.
Jiufu Qin,Jiufu Qin,Yongjin J. Zhou,Anastasia Krivoruchko,Mingtao Huang,Lifang Liu,Sakda Khoomrung,Verena Siewers,Bo Jiang,Jens Nielsen,Jens Nielsen +10 more
TL;DR: In this paper, a modular pathway rewiring (MPR) strategy was implemented for pathway optimization resulting in high-level production of L-ornithine, an intermediate of Larginine biosynthesis and a precursor metabolite for a range of different natural products.
Journal ArticleDOI
Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives
TL;DR: A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli and pyruvate was synthesized primarily via salicylate formation and the reaction converting oxaloacetate to pyruVate.
References
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Journal ArticleDOI
Opportunities and challenges for a sustainable energy future
Steven Chu,Arun Majumdar +1 more
TL;DR: This Perspective provides a snapshot of the current energy landscape and discusses several research and development opportunities and pathways that could lead to a prosperous, sustainable and secure energy future for the world.
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Osmotic Stress Signaling and Osmoadaptation in Yeasts
TL;DR: An integrated understanding of osmoadaptation requires not only knowledge of the function of many uncharacterized genes but also further insight into the time line of events, their interdependence, their dynamics, and their spatial organization as well as the importance of subtle effects.
Journal ArticleDOI
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
Eric J. Steen,Yisheng Kang,Gregory Bokinsky,Zhihao Hu,Andreas W. Schirmer,Amy McClure,Stephen B. del Cardayre,Jay D. Keasling +7 more
TL;DR: The engineering of Escherichia coli is demonstrated to produce structurally tailored fatty esters (biodiesel), fatty alcohols, and waxes directly from simple sugars, a step towards producing these compounds directly from hemicellulose, a major component of plant-derived biomass.
Journal ArticleDOI
Microbial Biosynthesis of Alkanes
TL;DR: The discovery of an alkane biosynthesis pathway in cyanobacteria that converts intermediates of fatty acid metabolism to alkanes and alkenes is described and is likely to be a valuable tool in the production of biofuels.
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