Journal ArticleDOI
Metabolic engineering of yeast for production of fuels and chemicals
TLDR
Recent scientific progress in metabolic engineering of S. cerevisiae for the production of bioethanol, advanced biofuels, and chemicals is reviewed.About:
This article is published in Current Opinion in Biotechnology.The article was published on 2013-06-01. It has received 292 citations till now.read more
Citations
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Journal ArticleDOI
Rewriting yeast central carbon metabolism for industrial isoprenoid production
Adam L. Meadows,Kristy Michelle Hawkins,Yoseph Tsegaye,Eugene Antipov,Youngnyun Kim,Lauren Raetz,Robert H. Dahl,Anna Tai,Tina Mahatdejkul-Meadows,Lan Xu,Lishan Zhao,Madhukar S. Dasika,Abhishek Murarka,Jacob R. Lenihan,Diana Eng,Joshua S. Leng,Chi-Li Liu,Jared W. Wenger,Hanxiao Jiang,Lily Chao,Patrick J. Westfall,Jefferson Lai,Savita Ganesan,Peter K. Jackson,Robert Mans,Darren Platt,Christopher D. Reeves,Poonam R. Saija,Gale Wichmann,Victor F. Holmes,Kirsten R. Benjamin,Paul W. Hill,Timothy S. Gardner,Annie Ening Tsong +33 more
TL;DR: Four non-native metabolic reactions are used to rewire central carbon metabolism in S. cerevisiae, enabling biosynthesis of cytosolic acetyl coenzyme A (acetyl-CoA, the two-carbon isoprenoid precursor) with a reduced ATP requirement, reduced loss of carbon to CO2-emitting reactions, and improved pathway redox balance.
Journal ArticleDOI
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.
Robert Mans,Harmen M. van Rossum,Melanie Wijsman,Antoon H. Backx,Niels G. A. Kuijpers,Marcel van den Broek,Pascale Daran-Lapujade,Jack T. Pronk,Antonius J. A. van Maris,Jean-Marc Daran +9 more
TL;DR: The versatility of CRISPR/Cas9-based engineering of yeast is demonstrated by achieving simultaneous integration of a multigene construct combined with gene deletion and the simultaneous introduction of two single-nucleotide mutations at different loci.
Journal ArticleDOI
Altered sterol composition renders yeast thermotolerant
Luis Caspeta,Yun Chen,Payam Ghiaci,Amir Feizi,Steen Buskov,Björn M. Hallström,Dina Petranovic,Jens Nielsen,Jens Nielsen +8 more
TL;DR: Adaptive laboratory evolution was used to select yeast strains with improved growth and ethanol production at ≥40°C, and a change in sterol composition was revealed, from ergosterol to fecosterol, caused by mutations in the C-5 sterol desaturase gene, and increased expression of genes involved in sterolsynthesis.
Journal ArticleDOI
Biological valorization of low molecular weight lignin
Omar Abdelaziz,Daniel P. Brink,Jens Prothmann,Krithika Ravi,Mingzhe Sun,Javier García-Hidalgo,Margareta Sandahl,Christian Hulteberg,Charlotta Turner,Gunnar Lidén,Marie F. Gorwa-Grauslund +10 more
TL;DR: A brief summary of sources of lignin, methods of depolymerization, biological pathways for conversion of the lign in monomers and the analytical tools necessary for characterizing and evaluating key lignIn attributes are given.
Journal ArticleDOI
Cell-free metabolic engineering: biomanufacturing beyond the cell.
TL;DR: In the coming years, CFME will offer exciting opportunities to debug and optimize biosynthetic pathways, carry out design‐build‐test iterations without re‐engineering organisms, and perform molecular transformations when bioconversion yields, productivities, or cellular toxicity limit commercial feasibility.
References
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Journal ArticleDOI
Engineering topology and kinetics of sucrose metabolism in Saccharomyces cerevisiae for improved ethanol yield
Thiago Olitta Basso,Thiago Olitta Basso,Stefan de Kok,Marcelo Goulart Dário,Marcelo Goulart Dário,Júlio Cézar A. do Espirito-Santo,Gabriela Muller,Paulo S. Schlölg,Carlos P. Silva,Aldo Tonso,Jean-Marc Daran,Andreas Karoly Gombert,Antonius J. A. van Maris,Jack T. Pronk,Boris U. Stambuk,Boris U. Stambuk +15 more
TL;DR: This study demonstrates that engineering the topology of sucrose metabolism is an attractive strategy to improve ethanol yields in industrial processes.
Journal ArticleDOI
Metabolome, transcriptome and metabolic flux analysis of arabinose fermentation by engineered Saccharomyces cerevisiae
H. Wouter Wisselink,Chiara Cipollina,Bart Oud,Barbara Crimi,Joseph J. Heijnen,Jack T. Pronk,Antonius J. A. van Maris +6 more
TL;DR: Elevated intracellular concentrations of pentose-phosphate-pathway intermediates and upregulation of TKL2 and YGR043c suggested an involvement of these genes in flux-controlling reactions in arabinose fermentation, which confirmed that the galactose transporter is essential for growth onArabinose.
Journal ArticleDOI
Systems biology of yeast: enabling technology for development of cell factories for production of advanced biofuels.
TL;DR: This review will cover the recent technological developments that support improvement of the advanced biofuels 1-butanol, biodiesels and jetfuels.
Journal ArticleDOI
Prospects for microbial biodiesel production
TL;DR: This work reviews both microbial approaches for renewable biodiesel production and evaluates the existing challenges in these two strategies.
Journal ArticleDOI
Improving L-arabinose utilization of pentose fermenting Saccharomyces cerevisiae cells by heterologous expression of L-arabinose transporting sugar transporters
Thorsten Subtil,Eckhard Boles +1 more
TL;DR: Using a newly developed screening system, two heterologous sugar transporters from a yeast and a plant are identified which can support uptake and utilization of L-arabinose in L-Arabinose fermenting S. cerevisiae cells, especially at low L-ARabinose concentrations.
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