Journal ArticleDOI
Metabolic engineering of yeast for production of fuels and chemicals
TLDR
Recent scientific progress in metabolic engineering of S. cerevisiae for the production of bioethanol, advanced biofuels, and chemicals is reviewed.About:
This article is published in Current Opinion in Biotechnology.The article was published on 2013-06-01. It has received 292 citations till now.read more
Citations
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Iterative algorithm-guided design of massive strain libraries, applied to itaconic acid production in yeast.
Eric M. Young,Zheng Zhao,Bianca Elisabeth Maria Gielesen,Liang Wu,D. Benjamin Gordon,Johannes Andries Roubos,Christopher A. Voigt,Christopher A. Voigt +7 more
TL;DR: Methods from design of experiments are applied to guide the construction of strain libraries from which the maximum information can be extracted without sampling every possible combination, revealing the optimal expression level of CAD as well as pairwise interdependencies between genes that result in increased titer and purity of itaconic acid.
Journal ArticleDOI
Cell-surface display of enzymes by the yeast Saccharomyces cerevisiae for synthetic biology
Tsutomu Tanaka,Akihiko Kondo +1 more
TL;DR: The recent progress of biorefinery fields in the development and application of yeast cell-surface displays from a synthetic biology perspective is summarized and approaches for further enhancing cell- surface display efficiency are discussed.
Journal ArticleDOI
The art of vector engineering: towards the construction of next-generation genetic tools.
Luísa Czamanski Nora,Cauã Antunes Westmann,Leonardo Martins-Santana,Luana de Fátima Alves,Lummy Maria Oliveira Monteiro,María-Eugenia Guazzaroni,Rafael Silva-Rocha +6 more
TL;DR: A detailed view following the evolution of vectors built throughout the years destined to study microorganisms and their peculiarities, including those whose genomes can only be revealed through metagenomics.
Journal ArticleDOI
A method for analysis and design of metabolism using metabolomics data and kinetic models: Application on lipidomics using a novel kinetic model of sphingolipid metabolism.
Georgios Savoglidis,Aline X.S. Santos,Isabelle Riezman,Paolo Angelino,Howard Riezman,Vassily Hatzimanikatis +5 more
TL;DR: A model-based method which can be used in conjunction with classical Metabolic Control Analysis for the analysis and design of cellular metabolism, and the class of enzymes regulating the distribution of sphingolipids among species and hydroxylation states is identified.
Journal ArticleDOI
Value-added biotransformation of cellulosic sugars by engineered Saccharomyces cerevisiae
TL;DR: This review summarizes recent advances in proficiency of engineering the yeast Saccharomyces cerevisiae for consuming lignocellulosic sugars, enabling the simultaneous assimilation of multiple carbon sources, and producing a large variety of value-added products by introduction of heterologous metabolic pathways.
References
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Journal ArticleDOI
Opportunities and challenges for a sustainable energy future
Steven Chu,Arun Majumdar +1 more
TL;DR: This Perspective provides a snapshot of the current energy landscape and discusses several research and development opportunities and pathways that could lead to a prosperous, sustainable and secure energy future for the world.
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Osmotic Stress Signaling and Osmoadaptation in Yeasts
TL;DR: An integrated understanding of osmoadaptation requires not only knowledge of the function of many uncharacterized genes but also further insight into the time line of events, their interdependence, their dynamics, and their spatial organization as well as the importance of subtle effects.
Journal ArticleDOI
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
Eric J. Steen,Yisheng Kang,Gregory Bokinsky,Zhihao Hu,Andreas W. Schirmer,Amy McClure,Stephen B. del Cardayre,Jay D. Keasling +7 more
TL;DR: The engineering of Escherichia coli is demonstrated to produce structurally tailored fatty esters (biodiesel), fatty alcohols, and waxes directly from simple sugars, a step towards producing these compounds directly from hemicellulose, a major component of plant-derived biomass.
Journal ArticleDOI
Microbial Biosynthesis of Alkanes
TL;DR: The discovery of an alkane biosynthesis pathway in cyanobacteria that converts intermediates of fatty acid metabolism to alkanes and alkenes is described and is likely to be a valuable tool in the production of biofuels.
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