Journal ArticleDOI
Metabolic engineering of yeast for production of fuels and chemicals
TLDR
Recent scientific progress in metabolic engineering of S. cerevisiae for the production of bioethanol, advanced biofuels, and chemicals is reviewed.About:
This article is published in Current Opinion in Biotechnology.The article was published on 2013-06-01. It has received 292 citations till now.read more
Citations
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Biotechnologie von Morgen: metabolisch optimierte Zellen für die bio‐basierte Produktion von Chemikalien und Treibstoffen, Materialien und Gesundheitsprodukten
Judith Becker,Christoph Wittmann +1 more
TL;DR: In this paper, a zielgerichtete Nutzbarmachung des grosen metabolischen Potentials durch den synergistischen Einsatz des Systems Metabolic Engineering and der chemischen Synthese verspricht neue and innovative Konzepte fur eine zukunftige Bio-Okonomie.
Journal ArticleDOI
Promoters inducible by aromatic amino acids and γ-aminobutyrate (GABA) for metabolic engineering applications in Saccharomyces cerevisiae
TL;DR: It is demonstrated that γ-aminobutyrate (GABA)-inducible UGA4 promoter, regulated by Uga3, can also be used in metabolic engineering as a dose-dependent inducible promoter.
Journal ArticleDOI
Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization
Sakda Khoomrung,José L. Martínez,Stefan Tippmann,Suwanee Jansa-Ard,Marieke F. Buffing,Raffaele Nicastro,Raffaele Nicastro,Jens Nielsen +7 more
TL;DR: In this article, an improved extraction and derivatization protocol for GC-MS analysis of amino/non-amino acids in Saccharomyces cerevisiae was presented.
Journal ArticleDOI
Incorporating comparative genomics into the design-test-learn cycle of microbial strain engineering.
TL;DR: It is discussed how incorporating comparative genomics into the design‐test‐learn cycle of strain engineering can provide novel information that complements other engineering strategies.
References
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Journal ArticleDOI
Opportunities and challenges for a sustainable energy future
Steven Chu,Arun Majumdar +1 more
TL;DR: This Perspective provides a snapshot of the current energy landscape and discusses several research and development opportunities and pathways that could lead to a prosperous, sustainable and secure energy future for the world.
Journal ArticleDOI
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Dae-Kyun Ro,Eric M. Paradise,Mario Ouellet,Karl Fisher,Karyn L. Newman,John M. Ndungu,Ho Kimberly,Eachus Rachel,Timothy S. Ham,James Kirby,Michelle C. Y. Chang,Sydnor T. Withers,Yoichiro Shiba,Richmond Sarpong,Jay D. Keasling +14 more
TL;DR: The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Journal ArticleDOI
Osmotic Stress Signaling and Osmoadaptation in Yeasts
TL;DR: An integrated understanding of osmoadaptation requires not only knowledge of the function of many uncharacterized genes but also further insight into the time line of events, their interdependence, their dynamics, and their spatial organization as well as the importance of subtle effects.
Journal ArticleDOI
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
Eric J. Steen,Yisheng Kang,Gregory Bokinsky,Zhihao Hu,Andreas W. Schirmer,Amy McClure,Stephen B. del Cardayre,Jay D. Keasling +7 more
TL;DR: The engineering of Escherichia coli is demonstrated to produce structurally tailored fatty esters (biodiesel), fatty alcohols, and waxes directly from simple sugars, a step towards producing these compounds directly from hemicellulose, a major component of plant-derived biomass.
Journal ArticleDOI
Microbial Biosynthesis of Alkanes
TL;DR: The discovery of an alkane biosynthesis pathway in cyanobacteria that converts intermediates of fatty acid metabolism to alkanes and alkenes is described and is likely to be a valuable tool in the production of biofuels.
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