Morpholino oligos: making sense of antisense?
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The evidence so far suggests that, with careful controls, morpholinos provide a relatively simple and rapid method to study gene function.About:
This article is published in Developmental Biology.The article was published on 2002-03-15 and is currently open access. It has received 596 citations till now. The article focuses on the topics: Morpholino.read more
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In vivo drug discovery in the zebrafish
TL;DR: By combining the scale and throughput of in vitro screens with the physiological complexity of animal studies, the zebrafish promises to contribute to several aspects of the drug development process, including target identification, disease modelling, lead discovery and toxicology.
Journal ArticleDOI
Zebrafish as a model vertebrate for investigating chemical toxicity.
TL;DR: An overview of the rapidly increasing use of zebrafish in toxicology is presented, both in identifying endpoints of toxicity and in elucidating mechanisms of toxicity.
Journal ArticleDOI
Antisense technologies. Improvement through novel chemical modifications.
TL;DR: Developing novel chemically modified nucleotides with improved properties such as enhanced serum stability, higher target affinity and low toxicity and the use of 21-mer double-stranded RNA molecules for RNA interference applications in mammalian cells offer highly efficient strategies to suppress the expression of a specific gene.
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siRNAs: applications in functional genomics and potential as therapeutics.
Yair Dorsett,Thomas Tuschl +1 more
TL;DR: Current nucleic-acid-based approaches for gene silencing are overviews, and the application of siRNAs in particular in functional genomics and as potential therapeutics is focused on.
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siRNAs : Applications in functional genomics and potential as therapeutics
Yair Dorsett,Thomas Tuschl +1 more
TL;DR: The use of small interfering RNA (siRNA) is one of the latest additions to the repertoire of sequence-specific gene-silencing agents as mentioned in this paper, and the robustness of this approach has motivated numerous biotechnology organizations and academic institutions to develop siRNA libraries for high-throughput genomewide screening in mammalian cells.
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Effective targeted gene ‘knockdown’ in zebrafish
TL;DR: It is shown here that antisense, morpholino-modified oligonucleotides (morpholinos) are effective and specific translational inhibitors in zebrafish, and conserved vertebrate processes and diseases are now amenable to a systematic, in vivo, reverse-genetic paradigm using zebra fish embryos.
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Morpholino antisense oligomers: design, preparation, and properties.
TL;DR: An overview of the design, preparation, and properties of Morpholino oligos, a novel antisense structural type that solves the sequence specificity problem and provides high and predictable activity in cells.
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Morpholino antisense oligomers: the case for an RNase H-independent structural type.
TL;DR: In cell-free and cultured-cell systems where one wishes to block the translation of a messenger RNA coding for a normal protein, RNase H-independent morpholino antisense oligos provide complete resistance to nucleases, generally good targeting predictability, generally high in-cell efficacy, excellent sequence specificity, and very preliminary results suggest they may exhibit little non-antisense activity.
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Overexpression of cadherins and underexpression of β-catenin inhibit dorsal mesoderm induction in early Xenopus embryos
Janet Heasman,Aaron Crawford,Kim Goldstone,Peggy Garner-Hamrick,Barry M. Gumbiner,Pierre D. McCrea,Chris Kintner,Chikako Yoshida Noro,Chris Wylie +8 more
TL;DR: Results show that beta-catenin has an important role in dorsal mesoderm induction and directly demonstrate the activity of a maternal mRNA in axis specification.
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βCatenin Signaling Activity Dissected in the Early Xenopus Embryo: A Novel Antisense Approach
TL;DR: The use of a novel antisense approach that allows for target depletion of protein to individual blastomeres and offers a rapid method for the functional analysis of both maternal and early zygotic gene products in Xenopus is reported.