Journal ArticleDOI
In vivo drug discovery in the zebrafish
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TLDR
By combining the scale and throughput of in vitro screens with the physiological complexity of animal studies, the zebrafish promises to contribute to several aspects of the drug development process, including target identification, disease modelling, lead discovery and toxicology.Abstract:
The zebrafish has become a widely used model organism because of its fecundity, its morphological and physiological similarity to mammals, the existence of many genomic tools and the ease with which large, phenotype-based screens can be performed. Because of these attributes, the zebrafish might also provide opportunities to accelerate the process of drug discovery. By combining the scale and throughput of in vitro screens with the physiological complexity of animal studies, the zebrafish promises to contribute to several aspects of the drug development process, including target identification, disease modelling, lead discovery and toxicology.read more
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Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish
Rupert J. Egan,Carisa L. Bergner,Peter C. Hart,Jonathan Cachat,Peter R. Canavello,Marco Elegante,Salem Elkhayat,Brett Bartels,Anna K. Tien,David Tien,Sopan Mohnot,Esther Beeson,Eric Glasgow,Hakima Amri,Zofia Zukowska,Allan V. Kalueff,Allan V. Kalueff +16 more
TL;DR: Zebrafish models of stress by analyzing how environmental and pharmacological manipulations affect their behavioral and physiological phenotypes are validated and alterations in whole-body cortisol levels in zebrafish parallel behavioral indices of anxiety are shown.
Journal ArticleDOI
Multi-target therapeutics: when the whole is greater than the sum of the parts
TL;DR: The biological rationale for combination therapeutics is discussed, some existing combination drugs are reviewed, and a systematic approach to identify interactions between molecular pathways that could be leveraged for therapeutic benefit is presented.
Journal ArticleDOI
In vivo imaging of transport and biocompatibility of single silver nanoparticles in early development of zebrafish embryos.
TL;DR: The transport of single silver nanoparticles into an in vivo model system (zebrafish embryos) and investigated their effects on early embryonic development at single-nanoparticle resolution in real time found that single Ag nanoparticles are transported into and out of embryos through chorion pore canals and exhibit Brownian diffusion (not active transport).
Journal ArticleDOI
Toxicity Assessments of Multisized Gold and Silver Nanoparticles in Zebrafish Embryos
TL;DR: It is proposed that nanoparticle chemistry is as, if not more, important than specific nanosizes at inducing toxicity in vivo and should lead to the identification of nanomaterial characteristics that afford minimal or no toxicity and guide more rational designs of materials on the nanoscale.
Journal ArticleDOI
The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish
TL;DR: These reporter lines will have utility in dissecting the genetic determinants of commitment to the myeloid lineage and in further defining how lysozyme-expressing cells participate during inflammation.
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Posted Content
The Innovator's Dilemma: When New Technologies Cause Great Firms to Fail
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