Multiple Sclerosis: MicroRNA Expression Profiles Accurately Differentiate Patients with Relapsing-Remitting Disease from Healthy Controls
Andreas Keller,Petra Leidinger,Julia Lange,Anne Borries,Hannah Schroers,Matthias Scheffler,Hans-Peter Lenhof,Klemens Ruprecht,Eckart Meese +8 more
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TLDR
The miRNA expression profiles in blood cells may serve as a biomarker for MS, and deregulation of mi RNA expression may play a role in the pathogenesis of MS.Abstract:
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system, which is heterogenous with respect to clinical manifestations and response to therapy. Identification of biomarkers appears desirable for an improved diagnosis of MS as well as for monitoring of disease activity and treatment response. MicroRNAs (miRNAs) are short non-coding RNAs, which have been shown to have the potential to serve as biomarkers for different human diseases, most notably cancer. Here, we analyzed the expression profiles of 866 human miRNAs. In detail, we investigated the miRNA expression in blood cells of 20 patients with relapsing-remitting MS (RRMS) and 19 healthy controls using a human miRNA microarray and the Geniom Real Time Analyzer (GRTA) platform. We identified 165 miRNAs that were significantly up- or downregulated in patients with RRMS as compared to healthy controls. The best single miRNA marker, hsa-miR-145, allowed discriminating MS from controls with a specificity of 89.5%, a sensitivity of 90.0%, and an accuracy of 89.7%. A set of 48 miRNAs that was evaluated by radial basis function kernel support vector machines and 10-fold cross validation yielded a specificity of 95%, a sensitivity of 97.6%, and an accuracy of 96.3%. While 43 of the 165 miRNAs deregulated in patients with MS have previously been related to other human diseases, the remaining 122 miRNAs are so far exclusively associated with MS. The implications of our study are twofold. The miRNA expression profiles in blood cells may serve as a biomarker for MS, and deregulation of miRNA expression may play a role in the pathogenesis of MS.read more
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Posted ContentDOI
Identifying common genes, proteins, and pathways from human miRNA and gene blood profiles in multiple sclerosis patients
TL;DR: In this paper , a novel approach has been taken for identifying genes associated with the disease and the top ten hub genes were used to identify drugs associated with them using EnrichR for enrichment analysis of genes.
The complex genetics of multiple sclerosis
TL;DR: This work has shown that microRNAs miR-17 and mir-20a inhibit T cell activation genes and are under-expressed in MS whole blood and candidate Gene SNP Association Studies in Multiple Sclerosis are candidate gene SNP Association studies.
Book ChapterDOI
MicroRNA regulation in autoimmune diseases
TL;DR: In this paper , a comprehensive overview of miRNA dysregulation in autoimmune diseases, highlighting their clinical significance as diagnostic markers and therapeutic targets is provided, which will be beneficial as biomarkers as well as novel targets for treating autoimmune diseases.
DissertationDOI
MicroRNA-Regulation in experimenteller autoimmuner Enzephalomyelitis im Vergleich mit Multiple Sklerose-Läsionen
TL;DR: In this article , the miRNA regulation of a selection of miRNAs was determined in EAE lesions of C57/BL6 mice (MOG35-55-induced) versus control and was compared to the known miRNA up-and down regulation in active human MS lesions.
Journal ArticleDOI
Cholecalciferol Supplementation Induced Up-Regulation of SARAF Gene and Down-Regulated miR-155-5p Expression in Slovenian Patients with Multiple Sclerosis
TL;DR: In this article , the authors investigated miRNA155-5p, a previously published pro-inflammatory miRNA in multiple sclerosis known to be correlated to cholecalciferol levels.
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