Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation
Shuling Zhou,Karolina Szczesna,Anna Ochalek,Julianna Kobolák,Eszter Varga,Csilla Nemes,Abinaya Chandrasekaran,Mikkel A. Rasmussen,Susanna Cirera,Poul Hyttel,Andras Dinnyes,Kristine K. Freude,Hasan X. Avci +12 more
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TLDR
It is determined that culturing iPSC-derived NPCs as three-dimensional (3D) floating neurospheres resulted in increased expression of the neural progenitor cell (NPC) markers, PAX6 and NESTIN, and the 3D propagation method could constitute a useful tool to promote NPC homogeneity and also to increase the differentiation potential of iPSCs towards astrocytes.Abstract:
Neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) are traditionally maintained and proliferated utilizing two-dimensional (2D) adherent monolayer culture systems. However, NPCs cultured using this system hardly reflect the intrinsic spatial development of brain tissue. In this study, we determined that culturing iPSC-derived NPCs as three-dimensional (3D) floating neurospheres resulted in increased expression of the neural progenitor cell (NPC) markers, PAX6 and NESTIN. Expansion of NPCs in 3D culture methods also resulted in a more homogenous PAX6 expression when compared to 2D culture methods. Furthermore, the 3D propagation method for NPCs resulted in a significant higher expression of the astrocyte markers GFAP and aquaporin 4 (AQP4) in the differentiated cells. Thus, our 3D propagation method could constitute a useful tool to promote NPC homogeneity and also to increase the differentiation potential of iPSC towards astrocytes.read more
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Astrocyte Differentiation of Human Pluripotent Stem Cells: New Tools for Neurological Disorder Research
TL;DR: The aim of this review article is to compare and summarize the currently available protocols and their strategies to generate human astrocytes from pluripotent stem cells (PSCs), and to discuss the potential role of human-induced PSCs derived astroCytes in disease modeling.
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Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine
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Genetic predispositions of Parkinson's disease revealed in patient-derived brain cells.
TL;DR: The interplay between genetic predispositions and midbrain neuronal impairments in people living with PD is discussed and the understanding of robust cellular phenotypes across genetic cohorts of Parkinson’s patients may guide future personalized drug screens in preclinical research.
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Comparison of 2D and 3D neural induction methods for the generation of neural progenitor cells from human induced pluripotent stem cells
Abinaya Chandrasekaran,Hasan X. Avci,Anna Ochalek,Lone N. Rösingh,Kinga Molnár,Lajos László,Tamás Bellák,Annamária Téglási,Krisztina Pesti,Arpad Mike,Phetcharat Phanthong,Orsolya Biró,Vanessa Jane Hall,Narisorn Kitiyanant,Karl-Heinz Krause,Julianna Kobolák,Andras Dinnyes +16 more
TL;DR: 3D neural induction increases the yield of PAX6+/NESTIN+ cells and gives rise to neurons with longer neurites, which might be an advantage for the production of forebrain cortical neurons, highlighting the potential of 3D Neural induction, independent of iPSCs' genetic background.
Journal ArticleDOI
Fast Generation of Functional Subtype Astrocytes from Human Pluripotent Stem Cells.
Xiang Li,Yezheng Tao,Robert A. Bradley,Zhongwei Du,Yunlong Tao,Linghai Kong,Yi Dong,Jeffrey P. Jones,Yuanwei Yan,Cole R. K. Harder,Lindsay Morgan Friedman,Magd Bilal,Brianna Hoffmann,Su-Chun Zhang +13 more
TL;DR: A simple and efficient method to efficiently generate astrocytes in 4–7 weeks by using CRISPR/Cas9-mediated inducible expression of NFIA or NFIA plus SOX9 in hPSCs and a strategy to generate region-specificAstrocyte subtypes by combining differentiation of regional progenitors and transgenic induction of astroCytes.
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