Neutrophil extracellular traps sequester circulating tumor cells and promote metastasis
Jonathan Cools-Lartigue,Jonathan Spicer,Braedon McDonald,Stephen Gowing,Simon C. Chow,Betty Giannias,Paul Kubes,Lorenzo E. Ferri +7 more
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TLDR
It is reported that circulating tumor cells become trapped within NETs in vitro under static and dynamic conditions and NETs are identified as potential therapeutic targets in the context of systemic infection.Abstract:
The majority of patients with cancer undergo at least one surgical procedure as part of their treatment. Severe postsurgical infection is associated with adverse oncologic outcomes; however, the mechanisms underlying this phenomenon are unclear. Emerging evidence suggests that neutrophils, which function as the first line of defense during infections, facilitate cancer progression. Neutrophil extracellular traps (NETs) are extracellular neutrophil-derived DNA webs released in response to inflammatory cues that trap and kill invading pathogens. The role of NETs in cancer progression is entirely unknown. We report that circulating tumor cells become trapped within NETs in vitro under static and dynamic conditions. In a murine model of infection using cecal ligation and puncture, we demonstrated microvascular NET deposition and consequent trapping of circulating lung carcinoma cells within DNA webs. NET trapping was associated with increased formation of hepatic micrometastases at 48 hours and gross metastatic disease burden at 2 weeks following tumor cell injection. These effects were abrogated by NET inhibition with DNAse or a neutrophil elastase inhibitor. These findings implicate NETs in the process of cancer metastasis in the context of systemic infection and identify NETs as potential therapeutic targets.read more
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Neutrophil extracellular traps (NETs) reduce the diffusion of doxorubicin which may attenuate its ability to induce apoptosis of ovarian cancer cells
Kohei Tamura,Hideyo Miyato,Rihito Kanamaru,Ai Sadatomo,Kazuya Takahashi,Hideyuki Ohzawa,Takahiro Koyanagi,Yasushi Saga,Yuji Takei,Hiroyuki Fujiwara,Alan T. Lefor,Naohiro Sata,Joji Kitayama +12 more
TL;DR: In this article , the authors examined how neutrophil extracellular traps (NETs) affect the pharmacokinetics and effects of doxorubicin (DOX).
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CXCR2 Mediates Distinct Neutrophil Behavior in Brain Metastatic Breast Tumor
TL;DR: It is shown that CXCR2 activation could be used by the brain metastatic tumors as a mechanism to program the tumor-infiltrating TANs into a pro-NETotic state, so as to assume a unique spatial distribution that assists in the subsequent migration and invasion of the metastatic tumor cells.
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Novel Cell Receptor System of Eukaryotes Formed by Previously Unknown Nucleic Acid-Based Receptors
Victor Tetz,George Tetz +1 more
TL;DR: The data provide the first evidence for the existence of a previously unknown receptive system formed by novel DNA- and RNA-based receptors in eukaryotes, named the TR-system, which is capable of recognizing and generating a response to different environmental factors and has been shown to orchestrate major vital functions of fungi, mammalian cells, and plants.
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The Role of Myeloid Cells in GBM Immunosuppression
TL;DR: The current advances in classifying epigenetic, metabolic, and phenotypic characteristics and functions of different populations of myeloid cells in glioma TME are detailed, as well as the mechanisms underlying promotion of tumorigenesis and immunosuppression.
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Histone H3 clipping is a novel signature of human neutrophil extracellular traps
Tilley Do,Ulrike Abu-Abed,Arndt Uz,Markus Schmid,Florian S,Peter R. Jungblut,Brinkmann,Alf Herzig,Arturo Zychlinsky +8 more
TL;DR: In this article, a histone histone H3 cleavage event was identified and harnessed to develop a site-specific antibody for the detection of human extracellular traps (NETs).
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