Neutrophil extracellular traps sequester circulating tumor cells and promote metastasis
Jonathan Cools-Lartigue,Jonathan Spicer,Braedon McDonald,Stephen Gowing,Simon C. Chow,Betty Giannias,Paul Kubes,Lorenzo E. Ferri +7 more
Reads0
Chats0
TLDR
It is reported that circulating tumor cells become trapped within NETs in vitro under static and dynamic conditions and NETs are identified as potential therapeutic targets in the context of systemic infection.Abstract:
The majority of patients with cancer undergo at least one surgical procedure as part of their treatment. Severe postsurgical infection is associated with adverse oncologic outcomes; however, the mechanisms underlying this phenomenon are unclear. Emerging evidence suggests that neutrophils, which function as the first line of defense during infections, facilitate cancer progression. Neutrophil extracellular traps (NETs) are extracellular neutrophil-derived DNA webs released in response to inflammatory cues that trap and kill invading pathogens. The role of NETs in cancer progression is entirely unknown. We report that circulating tumor cells become trapped within NETs in vitro under static and dynamic conditions. In a murine model of infection using cecal ligation and puncture, we demonstrated microvascular NET deposition and consequent trapping of circulating lung carcinoma cells within DNA webs. NET trapping was associated with increased formation of hepatic micrometastases at 48 hours and gross metastatic disease burden at 2 weeks following tumor cell injection. These effects were abrogated by NET inhibition with DNAse or a neutrophil elastase inhibitor. These findings implicate NETs in the process of cancer metastasis in the context of systemic infection and identify NETs as potential therapeutic targets.read more
Citations
More filters
Journal ArticleDOI
Neutrophil Extracellular Traps: Inflammation and Biomaterial Preconditioning for Tissue Engineering
Allison E. Fetz,Gary L. Bowlin +1 more
TL;DR: A tissue injury initiates a tissue repair program, characterized by acute inflammation and recruitment of immune cells, dominated by neutrophils as discussed by the authors, which prevent infection in the injured tissue.
Journal ArticleDOI
Circulating cell free DNA and citrullinated histone H3 as useful biomarkers of NETosis in endometrial cancer
Livia Ronchetti,Irene Terrenato,Margherita Ferretti,Giacomo Corrado,Frauke Goeman,Sara Donzelli,Chiara Mandoj,Roberta Merola,Ashanti Zampa,Mariantonia Carosi,Giovanni Blandino,Laura Monteiro de Castro Conti,Anna Maria Lobascio,Marcello Iacobelli,Enrico Vizza,Giulia Piaggio,Aymone Gurtner +16 more
TL;DR: In this article , the authors investigated NETosis biomarkers in endometrial cancer (EC) using qPCR and ELISA and found a correlation between increased levels of cfDNA, citH3 and inflammation features.
Journal ArticleDOI
NETworking with cancer: The bidirectional interplay between cancer and neutrophil extracellular traps.
TL;DR: In this paper , the authors discuss the bidirectional interplay by which cancer stimulates neutrophil extracellular traps (NETs), and NETs in turn support disease progression.
Journal ArticleDOI
The Heterogeneity of Neutrophil Recruitment in the Tumor Microenvironment and the Formation of Premetastatic Niches
TL;DR: In this paper, the authors discuss emerging research on the interaction between neutrophil recruitment and tumor metastasis, which is essential for studying tumor cell invasion and related immunotherapy, and discuss the interaction of neutrophils with tumor cells in distal metastasized tissues.
Journal ArticleDOI
Pharmacological inhibition of sodium-calcium exchange activates NADPH oxidase and induces infection-independent NETotic cell death
TL;DR: In this article, the authors showed that inhibition of forwardmode Na+/Ca2+ exchange by amiloride analogs, 5-(N-ethyl-N-isopropyl)amilorides (EIPA) and 5-(Methyl N-isobutyl)-amilodoride (MIA), triggers neutrophil extracellular traps (NETs) death independently of infectious stimuli.
References
More filters
Journal ArticleDOI
Global cancer statistics, 2002.
TL;DR: There are striking variations in the risk of different cancers by geographic area, most of the international variation is due to exposure to known or suspected risk factors related to lifestyle or environment, and provides a clear challenge to prevention.
Journal ArticleDOI
Cancer statistics, 2013
TL;DR: Overall cancer death rates have declined 20% from their peak in 1991 to 2009 and can be accelerated by applying existing cancer control knowledge across all segments of the population, with an emphasis on those groups in the lowest socioeconomic bracket and other underserved populations.
Journal ArticleDOI
Neutrophil extracellular traps kill bacteria
Volker Brinkmann,Ulrike Reichard,Christian Goosmann,Beatrix Fauler,Yvonne Uhlemann,David S. Weiss,Yvette Weinrauch,Yvette Weinrauch,Arturo Zychlinsky +8 more
TL;DR: It is described that, upon activation, neutrophils release granule proteins and chromatin that together form extracellular fibers that bind Gram-positive and -negative bacteria, which degrade virulence factors and kill bacteria.
Journal ArticleDOI
Revisions in the International System for Staging Lung Cancer
TL;DR: Analysis of a collected database representing all clinical, surgical-pathologic, and follow-up information for 5,319 patients treated for primary lung cancer confirmed the validity of the TNM and stage grouping classification schema.
Journal ArticleDOI
Preoperative Chemoradiotherapy for Esophageal or Junctional Cancer
TL;DR: Preoperative chemoradiotherapy improved survival among patients with potentially curable esophageal or esophagogastric-junction cancer and the regimen was associated with acceptable adverse-event rates.