NuA4-dependent Acetylation of Nucleosomal Histones H4 and H2A Directly Stimulates Incorporation of H2A.Z by the SWR1 Complex *
Mohammed Altaf,Andréanne Auger,Julie Monnet-Saksouk,Joëlle Brodeur,Sandra Piquet,Myriam Cramet,Nathalie Bouchard,Nicolas Lacoste,Rhea T. Utley,Luc Gaudreau,Jacques Côté +10 more
TLDR
The impact of NuA4-dependent acetylation on SWR1-driven incorporation of H2A.Z into chromatin is investigated, and depletion experiments indicate that the bromodomain-containing protein Bdf1 is important for NuA 4-dependent stimulation ofSWR1.About:
This article is published in Journal of Biological Chemistry.The article was published on 2010-05-21 and is currently open access. It has received 156 citations till now. The article focuses on the topics: Histone code & Histone exchange.read more
Citations
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Modification of Enhancer Chromatin: What, How, and Why?
Eliezer Calo,Joanna Wysocka +1 more
TL;DR: An overview of enhancer-associated modifications of histones and DNA is given and enzymatic activities involved in their dynamic deposition and removal are discussed and potential downstream effectors of these marks are described.
Journal ArticleDOI
Histone exchange, chromatin structure and the regulation of transcription
TL;DR: Ongoing research is elucidating the molecular mechanisms that regulate chromatin structure during transcription by preventing histone exchange, thereby limiting non-coding RNA expression.
Journal ArticleDOI
Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes
TL;DR: The 'hourglass' model of remodeller function is proposed, in which each remodeller subfamily utilizes diverse specialized proteins and protein domains to assist in nucleosomes targeting or to differentially detect nucleosome epitopes.
Journal ArticleDOI
Global regulation of H2A.Z localization by the INO80 chromatin remodeling enzyme is essential for genome integrity
TL;DR: Genetic interactions between ino80 and htz1 support a model in which INO80 catalyzes the removal of unacetylated H2A.Z from chromatin as a mechanism to promote genome stability.
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Stepwise histone replacement by SWR1 requires dual activation with histone H2A.Z and canonical nucleosome.
TL;DR: It is shown that promoter-proximal nucleosomes are highly heterogeneous for H2A.Z in Saccharomyces cerevisiae, with substantial representation of nucleosome containing one, two, or zero H2a.Z molecules.
References
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Chromatin Modifications and Their Function
TL;DR: The surface of nucleosomes is studded with a multiplicity of modifications that can dictate the higher-order chromatin structure in which DNA is packaged and can orchestrate the ordered recruitment of enzyme complexes to manipulate DNA.
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Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
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The Biology of Chromatin Remodeling Complexes
TL;DR: This work addresses many aspects of remodeler biology: their targeting, mechanism, regulation, shared and unique properties, and specialization for particular biological processes.
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Oscar Puig,Friederike Caspary,Guillaume Rigaut,Berthold Rutz,Emmanuelle Bouveret,Elisabeth Bragado-Nilsson,Matthias Wilm,Bertrand Séraphin +7 more
TL;DR: The TAP method is developed as a tool that allows rapid purification under native conditions of complexes, even when expressed at their natural level, and is a very useful procedure for protein purification and proteome exploration.
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Dynamic Regulation of Nucleosome Positioning in the Human Genome
Dustin E. Schones,Kairong Cui,Suresh Cuddapah,Tae-Young Roh,Artem Barski,Zhibin Wang,Gang Wei,Keji Zhao +7 more
TL;DR: It is found that nucleosome phasing relative to the transcription start sites is directly correlated to RNA polymerase II (Pol II) binding and the first nucleosomes downstream of a start site exhibits differential positioning in active and silent genes.