Journal ArticleDOI
Pluripotency and differentiation of cells from human testicular sperm extraction: An investigation of cell stemness.
Fatemeh Sadeghian-Nodoushan,Reza Aflatoonian,Zahra Borzouie,Fatemeh Akyash,Farzaneh Fesahat,Mehrdad Soleimani,Samaneh Aghajanpour,Harry Moore,Behrouz Aflatoonian +8 more
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TLDR
It was concluded that pluripotent stem cells could not be derived using the conditions previously reported to be successful for TESE samples, and multipotential differentiation capacity of these cells was confirmed.Abstract:
Human male germ-line stem cells (hmGSCs) and human testis-derived embryonic stem cell-like (htESC-like) cells are claimed to be in vitro pluripotent counterparts of spermatogonial stem cells (SSCs), but the origin and pluripotency of human testis-derived cell cultures are still under debate. The aim of this study was to generate putative pluripotent stem cells in vitro from human testicular sperm-extracted (TESE) samples of infertile men, and to assess their pluripotency and capacity to differentiate. TESE samples were minced, enzymatically disaggregated and dispersed into single-cell or cluster suspensions, and then cultured. Initially, cell clusters resembled those described for hmGSCs and htESC-like cells, and were positive for markers such as OCT4/POU5F1, NANOG, and TRA-2-54. Prolonged propagation of cell clusters expressing pluripotency markers did not thrive; instead, the cells that emerged possessed characteristics of mesenchymal stromal cells (MSCs) such as STRO-1, CD105/EGLN1, CD13/ANPEP, SOX9, vimentin, and fibronectin. KIT, SOX2, and CD44 were not expressed by these MSCs. The multipotential differentiation capacity of these cells was confirmed using Oil Red-O and Alizarin Red staining after induction with specific culture conditions. It is therefore concluded that pluripotent stem cells could not be derived using the conditions previously reported to be successful for TESE samples.read more
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Pluripotent Stem Cells in Adult Tissues: Struggling To Be Acknowledged Over Two Decades
TL;DR: Adult PSCs in adult tissues could be the ideal stem cell candidates to bring about endogenous regeneration compared to ES/iPS cells grown in Petri dish and also score better over ASCs which in fact are tissue committed progenitors with limited regenerative potential that differentiate from the P SCs.
Journal ArticleDOI
The influence of elastomeric polyurethane type and ratio on the physicochemical properties of electrospun polyurethane/silk fibroin hybrid nanofibers as potential scaffolds for soft and hard tissue engineering
Najmeh Dehghan-Manshadi,Saeid Fattahi,Mohsen Hadizadeh,Habib Nikukar,Seyed Mohammad Moshtaghioun,Seyed Mohammad Moshtaghioun,Behrouz Aflatoonian +6 more
TL;DR: In this paper, the hybrid fibrous scaffolds of two various types of aliphatic elastomeric polyurethane (EPU) and silk fibroin (SF) were fabricated with different mass ratio by electrospinning.
Journal ArticleDOI
Sertoli Cells Possess Immunomodulatory Properties and the Ability of Mitochondrial Transfer Similar to Mesenchymal Stromal Cells
Bianka Porubska,Bianka Porubska,Daniel Vasek,Veronika Somova,Michaela Hajkova,Michaela Hajkova,Michaela Hlaviznova,Tereza Tlapakova,Vladimir Holan,Vladimir Holan,Magdalena Krulova,Magdalena Krulova +11 more
TL;DR: It is suggested that SCs are promising candidates for application in regenerative medicine due to their anti-inflammatory and protective effects, especially in the therapies for diseases associated with testicular tissue inflammation.
Journal ArticleDOI
Attempts for Generation of Embryonic Stem Cells from Human Embryos Following In Vitro Embryo Twinning
TL;DR: In vitro embryo twinning by increasing the number of the human embryos could be useful in the future for the generation of new pluripotent stem cell lines, however, the challenge remains to optimize the methods.
Journal ArticleDOI
Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes.
Maryam Adib,Seyed Morteza Seifati,Mahmood Dehghani Ashkezari,Fatemeh Akyash,Arezoo Khoradmehr,Arezoo Khoradmehr,Behrouz Aflatoonian +6 more
TL;DR: The findings show that TCCM supports oocyte IVM in mice and affect oocyte morphology and hTCCM is an effective medium for GV oocyte growth and maturation compared to the control medium.
References
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Journal ArticleDOI
Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
Journal ArticleDOI
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Derivation of pluripotent stem cells from cultured human primordial germ cells
Michael J. Shamblott,Joyce Axelman,Shunping Wang,Elizabeth M. Bugg,John W. Littlefield,Peter J. Donovan,Paul D. Blumenthal,George R. Huggins,John D. Gearhart +8 more
TL;DR: In this article, a set of primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor (HILI) and forskolin.
Journal ArticleDOI
Reconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells
TL;DR: The generation of primordial germ cell-like cells (PGCLCs) in mice with robust capacity for spermatogenesis is demonstrated and provided a paradigm for the first step of in vitro gametogenesis.
Journal ArticleDOI
Derivation of oocytes from mouse embryonic stem cells
Karin Hübner,Guy Fuhrmann,Lane K. Christenson,James Kehler,Rolland Reinbold,Rabindranath De La Fuente,Jennifer R. Wood,Jerome F. Strauss,Michele Boiani,Hans R. Schöler +9 more
TL;DR: It is shown that mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop into blastocysts.