Quorum‐sensing autoinducer molecules produced by members of a multispecies biofilm promote horizontal gene transfer to Vibrio cholerae
TLDR
It is demonstrated that comEA transcription and the horizontal acquisition of DNA by V. cholerae are induced in response to purified CAI-1 and AI-2, and also by autoinducers derived from other Vibrios co-cultured with V. Cholerae within a mixed-species biofilm, suggesting that autoinducer communication within a consortium may promote DNA exchange among VibRIos.Abstract:
Vibrio cholerae, the causative agent of cholera and a natural inhabitant of aquatic environments, regulates numerous behaviors using a quorum-sensing (QS) system conserved among many members of the marine genus Vibrio. The Vibrio QS response is mediated by two extracellular autoinducer (AI) molecules: CAI-I, which is produced only by Vibrios, and AI-2, which is produced by many bacteria. In marine biofilms on chitinous surfaces, QS-proficient V. cholerae become naturally competent to take up extracellular DNA. Because the direct role of AIs in this environmental behavior had not been determined, we sought to define the contribution of CAI-1 and AI-2 in controlling transcription of the competence gene, comEA, and in DNA uptake. In this study we demonstrated that comEA transcription and the horizontal acquisition of DNA by V. cholerae are induced in response to purified CAI-1 and AI-2, and also by autoinducers derived from other Vibrios co-cultured with V. cholerae within a mixed-species biofilm. These results suggest that autoinducer communication within a consortium may promote DNA exchange among Vibrios, perhaps contributing to the evolution of these bacterial pathogens.read more
Citations
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Quadruple Quorum-Sensing Inputs Control Vibrio cholerae Virulence and Maintain System Robustness
TL;DR: This work shows that two other histidine kinases, CqsR and VpsS, act upstream in the central QS circuit of V. cholerae to activate LuxO, which is essential for virulence of this pathogen.
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Lateral gene transfer, bacterial genome evolution, and the Anthropocene.
TL;DR: This work creates hotspots for lateral transfer, such as wastewater systems, hospitals, and animal production facilities, and provides selective forces to fix such events in bacterial populations.
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Chitin colonization, chitin degradation and chitin-induced natural competence of Vibrio cholerae are subject to catabolite repression.
TL;DR: Results provided here indicate that cAMP and CRP are important in at least three interlinked areas of the chitin-induced natural competence programme.
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Interbacterial predation as a strategy for DNA acquisition in naturally competent bacteria
TL;DR: It is argued that the forced release of DNA from killed bacteria and the transfer of non-clonal genetic material have important roles in bacterial evolution.
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The Paradox of Mixed-Species Biofilms in the Context of Food Safety
TL;DR: It is proposed that novel and alternative techniques like antiquorum sensing, antibiofilms, enzymes, hurdle techniques, and bacteriophages will significantly help to control the formation of mixed-species biofilms for enhanced food safety.
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