Simultaneous Nasal Carriage by Methicillin-Resistant and Methicillin Susceptible Staphylococcus aureus of Lineage ST398 in a Live Pig Transporter.
Paula Gómez,Carmen Aspiroz,Nazreen F. Hadjirin,Daniel Benito,Myriam Zarazaga,Carmen Torres,Mark A. Holmes +6 more
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TLDR
The two nasal ST398 isolates recovered from the patient with previous occupational exposure to pigs appeared to have a livestock origin and could represent different evolutionary steps of animal-human interface lineage.Abstract:
Methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST)398 is a livestock associated (LA) lineage with zoonotic potential, especially in humans with live pig contact. The objective of this study was to characterize two S. aureus strains of lineage ST398 (one methicillin-resistant (MRSA), one methicillin-susceptible (MSSA)) isolated from the same nasal sample of a patient admitted in the Intensive-Care Unit of a Spanish Hospital, and with previous occupational exposure to live pigs, by whole-genome-sequencing (WGS). The sample was obtained during routine surveillance for MRSA colonization. Purified genomic DNA was sequenced using Illumina HiSeq 2000 and processed using conventional bioinformatics software. The two isolates recovered were both S. aureus t011/ST398 and showed similar resistance-phenotypes, other than methicillin susceptibility. The possession of antibiotic resistance genes was the same, except for the mecA-gene located in SCCmecV in the MRSA isolate. The MSSA isolate harbored remnants of a SCCmec following the deletion of 17342bp from a recombination between two putative primases. Both isolates belonged to the livestock-associated clade as defined by three canonical single-nucleotide-polymorphisms, and neither possessed the human immune evasion cluster genes, chp, scn, or sak. The core genome alignment showed a similarity of 99.6%, and both isolates harbored the same mobile genetic elements. The two nasal ST398 isolates recovered from the patient with previous occupational exposure to pigs appeared to have a livestock origin and could represent different evolutionary steps of animal-human interface lineage. The MSSA strain was formed as a result of the loss of the mecA gene from the livestock-associated-MRSA lineage.read more
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Transmission of antimicrobial resistance (AMR) during animal transport
Konstantinos Koutsoumanis,Ana Allende,Avelino Alvarez-Ordóñez,Declan Bolton,Sara Bover-Cid,Marianne Chemaly,Robert Davies,Alessandra De Cesare,Lieve Herman,Friederike Hilbert,Roland Lindqvist,Maarten Nauta,Giuseppe Ru,Marion Simmons,Panagiotis N. Skandamis,Elisabetta Suffredini,Héctor Argüello‐Rodríguez,Wietske Dohmen,Chiara Francesca Magistrali,Barbara Padalino,Bernd-Alois Tenhagen,John Threlfall,Raquel García-Fierro,Beatriz Guerra,Ernesto Liebana,Pietro Stella,Luísa Peixe +26 more
TL;DR: In this paper , the transmission of antimicrobial resistance (AMR) between food-producing animals (poultry, cattle and pigs) during short journeys and long journeys to other farms or to the slaughterhouse lairage (directly or with intermediate stops at assembly centres or control posts, mainly transported by road) was assessed.
Journal ArticleDOI
Clonal relatedness of coagulase-positive staphylococci among healthy dogs and dog-owners in Spain. Detection of multidrug-resistant-MSSA-CC398 and novel linezolid-resistant-MRSA-CC5
Idris Nasir Abdullahi,Carmen Lozano,Myriam Zarazaga,André Becker Simões Saidenberg,Marc Stegger,Carmen Torres +5 more
TL;DR: In this paper , the authors determined the nasal carriage rate of coagulase-positive staphylococci (CoPS) among healthy dogs and in-contact people, their genotypic characteristics and phylogenetic relatedness.
Journal ArticleDOI
How to survive pig farming: Mechanism of SCCmec element deletion and metabolic stress adaptation in livestock-associated MRSA
Charlotte A. Huber,Silver A. Wolf,Wilma Ziebuhr,Mark A. Holmes,Julia Assmann,Antina Lübke-Becker,Andrea Thürmer,Torsten Semmler,Julian Brombach,Astrid Bethe,Markus Bischoff,Lothar H. Wieler,Lennard Epping,Birgit Walther +13 more
TL;DR: In this paper , the authors performed in-depth genomic and transcriptomic analyses using the livestock-associated (LA-) sequence type (ST) 398, isolated from pigs and their local surroundings, indicated that differences between these methicillin resistant predecessors (MRSA) are often limited to the absence of the staphylococcal cassette chromosome mec (SCCmec) and few single nucleotide polymorphisms.
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