Journal ArticleDOI
Stearylated arginine-rich peptides: a new class of transfection systems.
Shiroh Futaki,Wakana Ohashi,Tomoki W. Suzuki,Miki Niwa,Seigo Tanaka,Kunihiro Ueda,Hideyoshi Harashima,Yukio Sugiura +7 more
TLDR
In the case of the Tat, FHV, and octaarginine peptides, N-terminal stearylation of the peptides increases the transfection efficiency by approximately 100 times to reach the same order of magnitude as that of LipofectAMINE, one of the most efficient commercially available transfections agents.About:
This article is published in Bioconjugate Chemistry.The article was published on 2001-10-27. It has received 470 citations till now. The article focuses on the topics: Lipofectamine & Membrane permeability.read more
Citations
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Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
Journal ArticleDOI
Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery
TL;DR: The different uptake pathways that are involved in nonviral gene delivery from a gene delivery point of view are reviewed and available knowledge concerning cellular entry and the intracellular trafficking of cationic lipid-DNA complexes (lipoplexes) and cationsic polymer- DNA complexes (polyplexes) is summarized.
PatentDOI
Cell Penetrating Peptides
Mattias Hällbrink,Margus Pooga,Madis Tallin Unive Metsis,Priit Kogerman,Andreas Valkna,Anne Meikas,Maria Lindgren,Astrid Gräslund,Göran Eriksson,Claes Göran Östensson,Metka V. Budihna,Matjaz Zorko,Anna Elmquist,Ursel Soomets,Pontus Lundberg,Peter Järver,Külliki Saar,Samir El-Andaloussi,Kalle Kilk,Ülo Langel +19 more
TL;DR: In this paper, a method for predicting or designing, detecting, and/or verifying a novel cell-penetrating peptide (CPP) was proposed and used for treating and preventing a medical condition.
Journal ArticleDOI
Cell-Penetrating Peptides : Design, Synthesis, and Applications
TL;DR: The intrinsic property of cell-penetrating peptides to deliver therapeutic molecules to cells and tissues in a nontoxic manner has indicated that they may be potential components of future drugs and disease diagnostic agents.
Journal ArticleDOI
Tat peptide-mediated cellular delivery: back to basics.
TL;DR: The mechanism by which the Tat peptide adheres to, and crosses, the plasma membrane of cells is currently a topic of heated discussion in the literature, with varied findings being reported.
References
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Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal ArticleDOI
A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus
TL;DR: The main determinants required for Tat translocation within this sequence are delineated by synthesizing several peptides covering the Tat domain from residues 37 to 60 and the domain extending from amino acid 37 to 47, which corresponds to the α-helix structure, is not required for cellular uptake and for nuclear translocation.
Journal ArticleDOI
The third helix of the Antennapedia homeodomain translocates through biological membranes
TL;DR: It is reported here that a polypeptide of 16 amino acids in length corresponding to the third helix of the homeodomain deleted of its N-terminal glutamate is still capable of translocating through the membrane, suggesting an energy-independent mechanism of translocation not involving classical endocytosis.
Journal ArticleDOI
Arginine-rich Peptides: AN ABUNDANT SOURCE OF MEMBRANE-PERMEABLE PEPTIDES HAVING POTENTIAL AS CARRIERS FOR INTRACELLULAR PROTEIN DELIVERY *
Shiroh Futaki,Tomoki Suzuki,Wakana Ohashi,Takeshi Yagami,Seigo Tanaka,Kunihiro Ueda,Yukio Sugiura +6 more
TL;DR: Based on the fluorescence microscopic observations of mouse macrophage RAW264.7 cells, it is found that various arginine-rich peptides have a translocation activity very similar to Tat-(48–60), and the results strongly suggested the possible existence of a common internalization mechanism ubiquitous to arkinine- rich peptides.
Journal ArticleDOI
Cell Internalization of the Third Helix of the Antennapedia Homeodomain Is Receptor-independent
Daniele Derossi,Sophie Calvet,Alain Trembleau,Alié Brunissen,Gérard Chassaing,Alain Prochiantz +5 more
TL;DR: The present demonstration, that a reverse helix and a helix composed of D-enantiomers still translocate across biological membranes at 4 and 37°C strongly suggests that the third helix of the homeodomain is internalized by a receptor-independent mechanism.