Journal ArticleDOI
Stem cell factor is encoded at the SI locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor
Krisztina M. Zsebo,David A. Williams,Edwin N. Geissler,Virginia C. Broudy,Francis Hall Martin,Harry L. Atkins,Rou Yin Hsu,Neal C. Birkett,Kenneth H. Okino,Murdock Douglas C,Frederick W. Jacobsen,Keith Langley,Kent A. Smith,Takashi Takeish,Bruce M. Cattanach,Stephen J. Galli,Sidney Vaughn Suggs +16 more
TLDR
In vivo administration of SCF can reverse the macrocytic anemia and locally repair the mast cell deficiency of Sl/Sld mice and provide biological and physical evidence that SCF is a ligand for the c-kit receptor.About:
This article is published in Cell.The article was published on 1990-10-05. It has received 1485 citations till now. The article focuses on the topics: Stem cell factor & Receptor tyrosine kinase.read more
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Patent
Methods for treating viral disorders
TL;DR: In this paper, methods of treating viral disorders via the administration of an inducing agent and an anti-viral agent were discussed, where the inducing agent is administered for about five days and the anti-veto agent is subsequently administered without the inducing agents for an additional period of about sixteen days for a total cycle of about 21 days.
Journal ArticleDOI
Cytofluorimetric and functional analysis of c-kit receptor in acute leukemia.
Francesco Lauria,Gian Paolo Bagnara,Damiano Rondelli,Donatella Raspadori,Pierluigi Strippoli,Laura Bonsi,M. A. Ventura,Lorenzo Montanaro,Giuliana Bubola,Sante Tura,Virginia C. Broudy +10 more
TL;DR: The SR-1 monoclonal antibody recognizes an epitope of the c-kit receptor (KR), present on normal hemopoietic CD34+ stem cells as well as on blasts from patients with acute leukemia, and "in vitro" cultures from 15 cases showed that recombinant human Stem cell factor induced an increased proliferative activity in most tested cases.
Journal ArticleDOI
Mutual Education Between Hematopoietic Cells and Bone Marrow Stromal Cells Through Direct Cell-to-Cell Contact: Factors That Determine the Growth of Bone Marrow Stroma-Dependent Leukemic (HB-1) Cells
Huijie Jiang,Kenkichi Sugimoto,Hitoshi Sawada,Emi Takashita,Maki Tohma,Hiroyuki Gonda,Kazuhiro Mori +6 more
TL;DR: Findings show the existence of two-way interactions between HB-1 and MS-10, and found that interleukin-1alpha (IL-1 alpha) produced by MS- 10 plays a major role in the survival and proliferation ofHB-1.
Short Communication Mutations in Exons 9 and 13 of KIT Gene Are Rare Events in Gastrointestinal Stromal Tumors
Jerzy Lasota,Agnieszka Wozniak,Maarit Sarlomo-Rikala,Janusz Rys,Radzisław Kordek,Aziza Nassar,Leslie H. Sobin,Markku Miettinen +7 more
TL;DR: Activating mutations in the juxtamembrane domain (exon 11) of the c-kit gene have been shown in a subset of GISTs, and these mutations lead into ligand-independent activation of the tyrosine kinase of c-Kit, and have a transforming effect in vitro.
Journal ArticleDOI
Implication of stem cell factor in the proliferation of choroidal melanocytes.
TL;DR: Investigation of the c-kit/stem cell factor pathway regulates the proliferation of choroidal melanocytes and also if such pathway plays a role in bilateral uveal melanocytic proliferation found a strong cytoplasmic distribution in the pigmented spindle-shaped melanocytes localized in the multiple focal areas of charoidal thickening.
References
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Journal ArticleDOI
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Journal ArticleDOI
Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.