Structure and function of the chromogranin A gene. Clues to evolution and tissue-specific expression.
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TLDR
The results suggest that this complex gene is encoded by exon modules with evolutionary links to homologous modules in other chromogranin/secretogran in protein family members, and that the 5'-flanking region of the gene is sufficient to confer neuroendocrine tissue-specific expression of the chromogrannin A gene.About:
This article is published in Journal of Biological Chemistry.The article was published on 1991-07-15 and is currently open access. It has received 110 citations till now. The article focuses on the topics: Chromogranin A & Coding region.read more
Citations
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The chromogranins A and B: The first 25 years and future perspectives
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Chromogranin A, an “On/Off” Switch Controlling Dense-Core Secretory Granule Biogenesis
TL;DR: It is concluded that CGA is an "on/off" switch that alone is sufficient to drive dense-core secretory granule biogenesis and hormone sequestration in endocrine cells.
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Novel autocrine feedback control of catecholamine release. A discrete chromogranin a fragment is a noncompetitive nicotinic cholinergic antagonist.
Sushil K. Mahata,Daniel T. O'Connor,Manjula Mahata,Seung Hyun Yoo,Laurent Taupenot,Hongjiang Wu,B. M. Gill,R J Parmer +7 more
TL;DR: This small domain within chromogranin A may contribute to a novel, autocrine, homeostatic (negative-feedback) mechanism controlling catecholamine release from chromaffin cells and neurons.
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The Extended Granin Family: Structure, Function, and Biomedical Implications
Alessandro Bartolomucci,Roberta Possenti,Sushil K. Mahata,Reiner Fischer-Colbrie,Y. Peng Loh,Stephen R.J. Salton +5 more
TL;DR: The structure and function of granins and granin-derived peptides and expansive new genetic evidence are reviewed, including recent single-nucleotide polymorphism mapping, genomic sequence comparisons, and analysis of transgenic and knockout mice, which together support an important and evolutionarily conserved role for these proteins in large dense-core vesicle biogenesis and regulated secretion.
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Tumor Markers in Neuroendocrine Tumors
TL;DR: At the moment, chromogranin A is considered the best general neuroendocrine serum or plasma marker available both for diagnosis and therapeutic evaluation and is increased in 50–100% of patients with various neuro endocrine tumors.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
TL;DR: A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.