Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays.
TLDR
A suite of complementary tests will inform crucial decisions by healthcare providers and policy makers and understanding their strengths and limitations will be critical to their judicious application for the development of algorithmic approaches to treatment and public health strategies.Abstract:
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated coronavirus disease 2019 (COVID-19) pandemic has demanded rapid upscaling of in-vitro diagnostic assays to enable mass screening and testing of high-risk groups, and simultaneous ascertainment of robust data on past SARS-CoV-2 exposure at an individual and a population level. To meet the exponential demand in testing, there has been an accelerated development of both molecular and serological assays across a plethora of platforms. The present review discusses the current literature on these modalities, including nucleic acid amplification tests, direct viral antigen tests and the rapidly expanding laboratory-based and point of care serological tests. This suite of complementary tests will inform crucial decisions by healthcare providers and policy makers, and understanding their strengths and limitations will be critical to their judicious application for the development of algorithmic approaches to treatment and public health strategies.read more
Citations
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COVID-19 Artificial Intelligence Diagnosis Using Only Cough Recordings
TL;DR: An AI speech processing framework that leverages acoustic biomarker feature extractors to pre-screen for COVID-19 from cough recordings, and provide a personalized patient saliency map to longitudinally monitor patients in real-time, non-invasively, and at essentially zero variable cost is developed.
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Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip.
TL;DR: An opto-microfluidic sensing platform with gold nanospikes, fabricated by electrodeposition, to detect the presence and amount of antibodies specific to the SARS-CoV-2 spike protein in human plasma diluted in 1 mL of buffer solution, demonstrating that this platform offers a promising point-of-care testing tool to complement standard serological assays and make Sars-Cov-2 quantitative diagnostics easier, cheaper, and faster.
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Antigen-Based Testing but Not Real-Time Polymerase Chain Reaction Correlates With Severe Acute Respiratory Syndrome Coronavirus 2 Viral Culture.
Andrew Pekosz,Valentin Parvu,Maggie Li,Jeffrey C Andrews,Yukari C. Manabe,Yukari C. Manabe,Salma Kodsi,Devin S. Gary,Celine Roger-Dalbert,Jeffry Leitch,Charles K. Cooper +10 more
TL;DR: In this paper, the correlation between SARS-CoV-2 antigen and SARS CoV2 culture positivity represents a significant advancement in determining the risk for potential transmissibility beyond that which can be achieved by detection of SARSCoV2 genomic RNA.
Journal ArticleDOI
Accuracy of novel antigen rapid diagnostics for SARS-CoV-2: A living systematic review and meta-analysis.
Lukas E. Brümmer,Stephan Katzenschlager,Mary Gaeddert,Christian Erdmann,Stephani Schmitz,Marc Bota,Maurizio Grilli,Jan Larmann,Markus A. Weigand,Nira R. Pollock,Aurélien Macé,Sergio Carmona,Stefano Ongarello,Jilian A. Sacks,Claudia M. Denkinger,Claudia M. Denkinger +15 more
TL;DR: A systematic review and meta-analysis of commercially available rapid diagnostic tests (Ag-RDTs) for SARS-CoV-2 up until 30 April 2021 was conducted in this paper.
Posted ContentDOI
The accuracy of novel antigen rapid diagnostics for SARS-CoV-2: a living systematic review and meta-analysis.
Lukas E. Brümmer,Stephan Katzenschlager,Mary Gaeddert,Christian Erdmann,Stephani Schmitz,Marc Bota,Maurizio Grilli,Jan Larmann,Markus A. Weigand,Nira R. Pollock,Sergio Carmona,Stefano Ongarello,Jilian A. Sacks,Claudia M. Denkinger +13 more
TL;DR: In this article, a systematic review and meta-analysis of commercially available rapid diagnostic tests (Ag-RDTs) is presented, where the clinical accuracy (sensitivity and specificity) of these tests are assessed.
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TL;DR: Results of an analysis of nasal and throat swabs from 17 patients in Zhuhai, China, who had received a diagnosis of Covid-19 and found SARS-CoV-2 Viral Load in Upper Respiratory Specimens positive.
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