Book ChapterDOI
The Bradford Method for Protein Quantitation
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TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
Citations
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Journal ArticleDOI
Role of lysine versus arginine in enzyme cold-adaptation: modifying lysine to homo-arginine stabilizes the cold-adapted alpha-amylase from Pseudoalteramonas haloplanktis.
Khawar Sohail Siddiqui,Anne Poljak,Michael Guilhaus,Davide De Francisci,Paul M. G. Curmi,Paul M. G. Curmi,Georges Feller,Salvino D'Amico,Charles Gerday,Vladimir N. Uversky,Vladimir N. Uversky,Ricardo Cavicchioli +11 more
TL;DR: Results indicate that replacing lysine with hR generates mesophilic‐like characteristics in AHA, and provides support for the importance of lysines residues in promoting enzyme cold adaptation, consistent with computational analyses that show that AHA possesses a compositional bias that favors decreased conformational stability and increased flexibility.
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Physical and Kinetic Properties of the Family 3 β-Glucosidase from Aspergillus niger Which Is Important for Cellulose Breakdown
TL;DR: A β-glucosidase purified from Aspergillus niger cellulase powder was characterized and inhibition experiments indicated that the enzyme is specific for glucosyl substrates and suggested that D-gluconolactone is a transition state analog.
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Effects of atrazine on the gill cells and ionic balance in a neotropical fish, Prochilodus lineatus
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Sunil Sahdeo,Brian D. Scott,Marissa Z. McMackin,Mittal Jasoliya,Brandon M. Brown,Heike Wulff,Susan Perlman,Mark A. Pook,Gino A Cortopassi +8 more
TL;DR: Dyclonine represents a novel therapeutic strategy that can potentially be repurposed for the treatment of FA and induces the Nrf2 [nuclear factor (erythroid-derived 2)-like 2] transcription factor, which shows binds an upstream response element in the FXN locus.
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iTRAQ-Based Quantitative Proteomic Analysis of Cotton Roots and Leaves Reveals Pathways Associated with Salt Stress.
Chen Tingting,Zhang Lei,Haihong Shang,Shaodong Liu,Peng Jun,Gong Wankui,Shi Yuzhen,Siping Zhang,Junwen Li,Juwu Gong,Ge Qun,Liu Aiying,Huijuan Ma,Xinhua Zhao,Youlu Yuan +14 more
TL;DR: A proteomic analysis of cotton roots and leaf tissue following exposure to saline stress concluded that the phenylalanine metabolism and starch and sucrose metabolism were active for energy homeostasis to cope with salt stress in cotton roots.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.