Book ChapterDOI
The Bradford Method for Protein Quantitation
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TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
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Bioethanol and biobutanol production from sugarcorn juice
Reyna Gomez-Flores,Thirumalai Nambi Thiruvengadathan,Robert W. Nicol,Brandon H. Gilroyed,Malcolm J. Morrison,Lana M. Reid,Argyrios Margaritis +6 more
TL;DR: For the first time, the authors used juice extracted from sugarcorn plants for bioethanol and biobutanol production via microbial fermentation, which achieved a butanol concentration of 8.3% in 257h (yield = 0.31% butanol per g total fermentable sugars).
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Novel phytase from Pteris vittata resistant to arsenate, high temperature, and soil deactivation.
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Metabolic modulation of redox state confounds fish survival against Vibrio alginolyticus infection.
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Green Synthesis of Gold and Silver Nanoparticles Using Leaf Extract of Capsicum chinense Plant
Diego Alberto Lomelí-Rosales,Adalberto Zamudio-Ojeda,Oscar Kevin Reyes-Maldonado,Morelia Lopez‐Reyes,Georgina Cristina Basulto-Padilla,Edgar J. López-Naranjo,Victor M. Zúñiga-Mayo,Gilberto Velázquez-Juárez +7 more
TL;DR: In this article , aqueous extracts of root, stem and leaf of Capsicum chinense was evaluated, obtaining the best results with the leaf extract, and gold and silver nanoparticles synthesized using leaf extract were characterized by UV-visible spectrophotometry (UV-Vis), Fourier Transform Infrared Spectroscopy with Attenuated Total Reflection (FTIR-ATR), X-ray Photoelectron Spectrogram (XPS), Ultra Hight Resolution Scanning Electron Microscopy coupled to Energy-Dispersive Xray spectroscopy (UHR-SEM-EDX) and Transmission Electron microscopy (TEM), and tested for their antioxidant and antimicrobial activities.
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Focal Adhesion Kinase Knockdown in Carcinoma-Associated Fibroblasts Inhibits Oral Squamous Cell Carcinoma Metastasis via Downregulating MCP-1/CCL2 Expression
Anjie Min,Chao Zhu,Jing Yi Wang,Jing Yi Wang,Shuping Peng,Cijun Shuai,Shan Gao,Shan Gao,Zhangui Tang,Tong Su +9 more
TL;DR: The results showed that oral CAFs expressed a higher level of FAK than normal human gingival fibroblasts, and the conditioned medium of CAFs could induce the invasion and migration of SCC‐25, one oral squamous carcinoma cell line, indicating that targeting FAK in CAFs might be a promising strategy for the treatment of OSCC in the future.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.