Book ChapterDOI
The Bradford Method for Protein Quantitation
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TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
Citations
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Journal ArticleDOI
The influence of LOX-less barley malt on the flavour stability of wort and beer
Yu Junhong,Huang Shuxia,Jianjun Dong,Wei Fan,Huang Shuli,Liu Jia,Zongming Chang,Yu-Hong Tian,Junguang Hao,Hu Shumin +9 more
TL;DR: This paper investigated the influence of lipoxygenase-less (LOX-less) barley malt on the quality of wort and beer, with the main focus on beer flavour stability.
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Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects.
TL;DR: Three most upregulated heat shock response genes (CPR6, FES1, and STI1) were co-overexpressed in K. phaffii and proved their positive effect on the secretion of reporter enzymes (PLA2 and prolyl endopeptidase) by increasing the production up to 1.41-fold, providing novel helper factors for rational engineering of K.phaffii.
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Proteins and their derived peptides as carriers in a conjugate vaccine for Streptococcus pneumoniae: self-heat shock protein 60 and tetanus toxoid.
TL;DR: In this paper, self and foreign peptides and their source proteins conjugated to the capsular polysaccharide (CPS) of type 4 Pn were used to induce T cell help for vaccination against Streptococcus pneumoniae.
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Evaluation of circulating cellular DCLK1 protein, as the most promising colorectal cancer stem cell marker, using immunoassay based methods.
Alireza Mirzaei,Zahra Madjd,Azade Amini Kadijani,Masoumeh Tavakoli-Yaraki,Mohammad Hossein Modarresi,Javad Verdi,Abolfazl Akbari,Gholamreza Tavoosidana +7 more
TL;DR: Evaluating CCDP in the peripheral blood mononuclear cells of colorectal cancer patients applying immunoassay based methods including PLA, IPCR and ELISA found ELISA could be the most judicious method for clinical evaluation of CCDP considering its simplicity for clinical implications.
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Amyloid beta and diabetic pathology cooperatively stimulate cytokine expression in an Alzheimer's mouse model
Sitara B. Sankar,Carmen Infante-Garcia,Laura D. Weinstock,Juan Jose Ramos-Rodriguez,Juan Jose Ramos-Rodriguez,Carmen Hierro-Bujalance,Cecilia Fernández-Ponce,Levi B. Wood,Levi B. Wood,Monica Garcia-Alloza +9 more
TL;DR: It is shown that Alzheimer’s and diabetic pathologies cooperate to enhance profiles of cytokines reported to be involved in both diseases, and strategies targeting neuroinflammatory signaling and metabolic control may provide a promising strategy for intervening in the development of diabetes-associated AD.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.