Book ChapterDOI
The Bradford Method for Protein Quantitation
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TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
Citations
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Preparation of chitosan self-aggregates as a gene delivery system.
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Book ChapterDOI
Biomass and abundance
L. Postel,H. Fock,W. Hagen +2 more
TL;DR: In this article, the authors proposed two principles related to the volumetric methods: the direct volume estimation of settled plankton and the measurement of the amount of water displaced by the plankton.
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At‐HSP17.6A, encoding a small heat‐shock protein in Arabidopsis, can enhance osmotolerance upon overexpression
TL;DR: The gene that encoded the cytosolic class II smHSP in Arabidopsis thaliana was characterized and its expression was induced by heat and osmotic stress, as well as during seed development, suggesting stress-induced post-transcriptional regulation of At-HSP17.6A expression.
Journal ArticleDOI
Cytokine Kinetics and Other Host Factors in Response to Pneumococcal Pulmonary Infection in Mice
Yves Bergeron,Nathalie Ouellet,Anne-Marie Deslauriers,Marie Simard,Martin Olivier,Michel G. Bergeron +5 more
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Comparison of Permanganate Preoxidation and Preozonation on Algae Containing Water: Cell Integrity, Characteristics, and Chlorinated Disinfection Byproduct Formation
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References
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Journal ArticleDOI
Adaptation of the dye-binding protein assay to microtiter plates.
TL;DR: The dye-binding protein assay has been adapted for use with microtiter plates and a plate reader, and the response of nitrate reductase in the assay is about 70% of that of the standard protein, bovine serum albumin.
Journal ArticleDOI
Assay for protein by dye binding.
Harold Van Kley,Susan M. Hale +1 more
TL;DR: A proposed assay for protein by binding of Coomassie brilliant blue G-250 has been evaluated and some proteins give standard curves similar to those reported by Bradford, but others deviate widely.
Journal ArticleDOI
Interference by lipids in the determination of protein using bicinchoninic acid
TL;DR: The results indicate that the presence of appreciable quantities of lipid in samples can cause significant error in the analysis of protein by the bicinchoninic acid procedure.
Journal ArticleDOI
Sensitivity and variability of the Bradford protein assay in the presence of detergents.
TL;DR: Triton X-100 appears to facilitate nonionic interactions of the dye with proteins of limited capacity for ionic binding, and the incorporation of low concentrations of a nonionic detergent may be useful in improving sensitivity and variability of the Bradford assay.
Journal ArticleDOI
A spectral study of the charge forms of Coomassie blue G
TL;DR: The conclusion is drawn that the assay conditions have been well optimized by earlier workers, and the potential for improvement of the CBB protein assay is discussed.