Book ChapterDOI
The Bradford Method for Protein Quantitation
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TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
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Glomalin-Related Soil Protein Reflects the Heterogeneity of Substrate and Vegetation in the campo rupestre Ecosystem
TL;DR: In this article, the authors examined whether glomalin-related soil protein (GRSP), produced by arbuscular mycorrhizal fungi (AMF), varies in relation to substrate and plant community in the campo rupestre ecosystem.
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A Flowthrough Assay for Rapid Bedside Stratification of Bloodstream Bacterial Infection in Critically Ill Patients: a Pilot Study.
TL;DR: The high sensitivity, low cost, and simple bedside utility of the assay may aid in better sepsis management apparently at the presymptomatic stage, lowering empirical therapy, medical costs, antimicrobial resistance, and mortality.
Journal ArticleDOI
Expression and characterization of 1,4-α-glucan branching enzyme from Microvirga sp. MC18 and its application in the preparation of slowly digestible starch
Xianfeng Ye,Wei Liu,Shiyun Ma,Xiaopei Chen,Yan Qiao,Yuqiang Zhao,Qiwen Fan,Xu Li,Chaonan Dong,Xiaodong Fang,Minghui Huan,Jian Han,Yan Huang,Zhongli Cui,Zhoukun Li +14 more
TL;DR: In this paper, the glucan branching enzyme McGBE from Microvirga sp. MC18 was identified, and its relevant properties as well as its potential for industrial starch modification were evaluated.
Journal ArticleDOI
Molecular cloning and functional characterization of a Cu/Zn superoxide dismutase from jellyfish Cyanea capillata.
Bo Wang,Guoyan Liu,Chao Wang,Zengliang Ruan,Qianqian Wang,Beilei Wang,Leilei Qiu,Shuaijun Zou,Xiping Zhang,Liming Zhang +9 more
TL;DR: The results suggest that CcSOD1 protein may play an important role in protecting jellyfish from oxidative damage and can serve as a new resource for antioxidant products.
Journal ArticleDOI
A high quality method for hemolymph collection from honeybee larvae.
Nicole Pavan Butolo,Patricia Azevedo,Luciano Delmondes de Alencar,Caio Eduardo da Costa Domingues,Lucas Miotelo,Osmar Malaspina,Roberta Cornélio Ferreira Nocelli,Roberta Cornélio Ferreira Nocelli +7 more
TL;DR: The present work established a high quality and easily reproducible method of extracting hemolymph from honeybee larvae (Apis mellifera), the extraction with ophthalmic scissors, and turbidity analyses of the samples using a turbidimeter showed that the use of ophthalic scissors provided the clearest samples and was free from contamination.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.