Book ChapterDOI
The Bradford Method for Protein Quantitation
Reads0
Chats0
TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
Citations
More filters
Journal ArticleDOI
Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell
Shahrul Hisham Zainal Ariffin,Nur Akmal Mohamed Rozali,Rohaya Megat Abdul Wahab,Sahidan Senafi,Intan Zarina Zainol Abidin,Zaidah Zainal Ariffin +5 more
TL;DR: The data suggested that α-MEM supplemented with 10 % NBCS is the best media for the expansion of both mouse and human suspension PBMNSCs, as supported by the significant increase in ALP specific activity.
Journal ArticleDOI
Cytoglobin protects cancer cells from apoptosis by regulation of mitochondrial cardiolipin.
Lorna Susan Thorne,Garret Rochford,Tim D. Williams,Andrew D. Southam,Giovanny Rodriguez-Blanco,Warwick B. Dunn,Nikolas J. Hodges +6 more
TL;DR: In this paper, the authors developed a new cell model to study the function of cytoglobin in oral squamous carcinoma and response to cisplatin and showed that cytoglobin mediated changes in expression of genes related to stress response, redox metabolism, mitochondrial function, cell adhesion, and fatty acid metabolism.
Journal ArticleDOI
Evaluation of temperature, pH and nutrient conditions in bacterial growth and extracellular hydrolytic activities of two Alicyclobacillus spp. strains
Lourdes Yaret Ortiz-Cortés,Lucía María Cristina Ventura-Canseco,Miguel Abud-Archila,Víctor Manuel Ruíz-Valdiviezo,Irving Oswaldo Velázquez-Ríos,Peggy Elizabeth Álvarez-Gutiérrez +5 more
TL;DR: Alicyclobacillus sp. PA1 and PA2 were isolated from crater lake El Chichon in Chiapas, Mexico as discussed by the authors, and the two strains were Gram variable, and colonies were circular, smooth and creamy.
Journal ArticleDOI
A COMPARATIVE EVALUATION OF SALIVARY FLOW RATE, pH, BUFFERING CAPACITY, CALCIUM AND TOTAL PROTEIN LEVELS IN PREGNANT AND NON PREGNANT WOMEN
Shweta Hegde,Neha Singh Thakur,Shweta Kohli,Vandana Shukla,Amen Siddiqui,Prakash Patel,Sangeeta Payasi,Sudhir Hegde,Kohli S,Shukla,Ammar Ahmed Siddiqui,P. K. Patel +11 more
TL;DR: Salivary flow rate, pH has decreased in pregnancy than non pregnant women, and DMFT index showed a strong correlation with pH in pregnant women and nonpregnant women, but difference was statistically non significant.
Journal ArticleDOI
Follicle-stimulating hormone-mediated decline in miR-92a-3p expression in pubertal mice Sertoli cells is crucial for germ cell differentiation and fertility
Alka Gupta,Amandeep Vats,A. Ghosal,Kamal Mandal,Rajesh Kumar Sarkar,Indrashis Bhattacharya,Sanjeev Das,Rahul Pal,Subeer S. Majumdar +8 more
TL;DR: In vivo observations provided substantial evidence that FSH-induced decline in miR-92a-3p expression during Sc maturation acts as an essential prerequisite for the pubertal onset of spermatogenesis.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.