Book ChapterDOI
The Bradford Method for Protein Quantitation
Reads0
Chats0
TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
Citations
More filters
Journal ArticleDOI
Immunotoxicity of silicon dioxide nanoparticles with different sizes and electrostatic charge
Jae Hyun Kim,Cheol-Su Kim,Rosa Mistica C. Ignacio,Dong Heui Kim,Ma Easter Joy Sajo,Eun Ho Maeng,Xu Feng Qi,Seong Eun Park,Yu Ri Kim,Meyoung Kon Kim,Kyu-Jae Lee,Soo Ki Kim +11 more
TL;DR: Interestingly, the small-sized and negatively charged SiO2 NPs showed the most potent in vivo immunotoxicity by way of suppressing the proliferation of lymphocytes, depressing the killing activity of NK cells, and decreasing proinflammatory cytokine production, thus leading to immunosuppression.
Journal ArticleDOI
Biomimetic modified clinical-grade POSS-PCU nanocomposite polymer for bypass graft applications: a preliminary assessment of endothelial cell adhesion and haemocompatibility.
Atefeh Solouk,Brian G. Cousins,Fereshteh Mirahmadi,Hamid Mirzadeh,Mohammadreza Jalali Nadoushan,Mohammad Ali Shokrgozar,Alexander M. Seifalian +6 more
TL;DR: Poly-AA-g-POSS-PCU surfaces offer binding sites for the covalent bonding of collagen type I and other biomolecules such as fibronectin by exposure of RGD cell binding domains and growth factors using EDC cross-linking chemistry.
Journal ArticleDOI
Two-dimensional gel electrophoresis in the light of new developments
TL;DR: Two-dimensional gel electrophoresis has become the most widely used separation tool in proteomic analysis and is gaining popularity due to the possibility of coupling it with numerous analytical techniques and bioinformatics methods.
Journal ArticleDOI
Titanium Dioxide Nanoparticles Alleviate Tetracycline Toxicity to Arabidopsis thaliana (L.)
Hong Liu,Hong Liu,Chuanxin Ma,Chuanxin Ma,Guangcai Chen,Jason C. White,Zonghua Wang,Baoshan Xing,Om Parkash Dhankher +8 more
TL;DR: In this article, the authors investigated the biochemical and molecular response upon coexposures to tetracycline (TC) and titanium oxide nanoparticles (TiO2 NPs).
Journal ArticleDOI
Comparison of sodium carbonate-oxygen and sodium hydroxide-oxygen pretreatments on the chemical composition and enzymatic saccharification of wheat straw.
TL;DR: The delignification efficiency and total sugar yield from enzymatic hydrolysis were comparable to the previously reported results at much higher temperature without oxygen.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.