Book ChapterDOI
The Bradford Method for Protein Quantitation
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TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
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Discovery of highly conserved unique peanut and tree nut peptides by LC-MS/MS for multi-allergen detection.
TL;DR: Two peptides were selected to represent peanut, almond, pecan, cashew, walnut, hazelnut, pine nut, Brazil nut, macadamia nut, pistachio nut, chestnut and coconut to determine the presence of trace levels of peanut and tree nuts in food by a novel multiplexed LC-MS method.
Journal ArticleDOI
Route of vaccine administration: effects on the specific humoral response in rainbow trout Oncorhynchus mykiss.
TL;DR: The data support the possibility that teleosts, like higher vertebrates, have a protective immune response to extracellular bacteria that is predominantly humoral, and suggest that route of delivery may primarily affect the efficiency with which the immunogenic constituents of the vaccine are presented to the relevant recognition and effector components of the immune system.
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Partitioning of protease from stomach of albacore tuna (Thunnus alalunga) by aqueous two-phase systems
TL;DR: ATPS can be effectively used to recover and purify protease from albacore tuna stomach and might be activated to pepsin by acidification and partitioning process.
Journal ArticleDOI
Ohr (Organic Hydroperoxide Resistance Protein) Possesses a Previously Undescribed Activity, Lipoyl-dependent Peroxidase
José Renato Rosa Cussiol,Thiago Geronimo Pires Alegria,Luke I. Szweda,Luis Eduardo Soares Netto +3 more
TL;DR: It is shown that lipoylated enzymes present in the bacterial extracts of Xylella fastidiosa interacted physically and functionally with this Cys-based peroxidase, whereas thioredoxin and glutathione systems failed to support Ohr peroxIDase activity.
Journal ArticleDOI
MG132 proteasome inhibitor modulates proinflammatory cytokines production and expression of their receptors in U937 cells: involvement of nuclear factor‐κB and activator protein‐1
Pablo C Ortiz-Lazareno,Georgina Hernández-Flores,Jorge R. Dominguez-Rodriguez,José Manuel Lerma-Díaz,Luis Felipe Jave-Suárez,Adriana Aguilar-Lemarroy,Piedad C. Gomez-Contreras,Daniel Scott-Algara,Alejandro Bravo-Cuellar,Alejandro Bravo-Cuellar +9 more
TL;DR: In this study, the human monocyte cell line U937 stimulated with lipopolysaccharide and phorbol 12‐myristate 13‐acetate as a model is used to investigate the in vitro effects of MG132, a proteasome inhibitor, on the release of proinflammatory cytokines and their receptors and on the expression of their membrane and soluble receptors.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.