Book ChapterDOI
The Bradford Method for Protein Quantitation
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A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
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Thermal stability and rheological properties of heat-induced gels prepared using edible insect proteins in a model system
TL;DR: In this paper, the thermal stability and rheological properties of heat-induced gels prepared using edible insect proteins originating from various edible insect species: Tenebrio molitor L, Protaetia brevitarsis larvae, and Allomyrina dichotoma larvae.
Journal ArticleDOI
Proteomics analysis reveals the role of ubiquitin specific protease (USP47) in Epithelial to Mesenchymal Transition (EMT) induced by TGFβ2 in breast cells.
Virgínia Campos Silvestrini,Carolina Hassibe Thomé,Daniele Albuquerque,Camila de Souza Palma,Germano Aguiar Ferreira,Guilherme Pauperio Lanfredi,Ana Paula Masson,Lara Elis Alberici Delsin,Fernanda Ursoli Ferreira,Felipe Canto de Souza,Lyris M. F. de Godoy,Adriano Aquino,Emanuel Carrilho,Rodrigo Alexandre Panepucci,Dimas Tadeu Covas,Vitor M. Faça +15 more
TL;DR: A model of EMT induction by TGFβ2 mimics early stage of metastatic cancer in epithelial breast cells and a proteomic study carried out for such model demonstrates that the deubiquitinase enzyme USP47 acts in SNAIL stabilization, one of the most important transcription factors for EMT phenotype acquisition and consequent metastasis.
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Evaluation of antioxidant properties of phycobiliproteins and phenolic compounds extracted from Bangia atropurpurea
TL;DR: The presence of R-phycoerythrin, phycocyanin and phenolic compounds in an array, amplifies the antioxidant property of B. atropurpurea.
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Maternal investment and nutrient use affect phenotype of American alligator and domestic chicken hatchlings.
TL;DR: Investigation of egg composition, embryo growth, and nutrient use in the domestic chicken and American alligator found variation in maternal investment of yolk at oviposition was responsible for nearly all of the variation in alligator hatchling phenotype, while both yolk and albumen contributed to chicken hatchling mass.
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TLR4-HMGB1 signaling pathway affects the inflammatory reaction of autoimmune myositis by regulating MHC-I.
TL;DR: The TLR4-HMGB1 signaling pathway affects the process of autoimmune myositis inflammation by regulating the expression of MHC-I and other pro-inflammatory cytokines.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.