Book ChapterDOI
The Bradford Method for Protein Quantitation
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TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
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PTH(7-84) inhibits PTH(1-34)-induced 1,25-(OH)2D3 production in murine renal tubules.
TL;DR: In vitro data suggest that PTH(7-84) elicits an antagonistic effect in renal tubules through receptors different from the type I PTH/PTHrP receptor, which may at least partly account for the decreased serum level of 1,25-(OH)2D in patients with severe primary hyperparathyroidism with renal failure.
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Transcription factor Hap5 induces gsh2 expression to enhance 2-phenylethanol tolerance and production in an industrial yeast Candida glycerinogenes.
TL;DR: It is discovered that Hap5 is an essential regulator to 2- PE resistance, and its induction by 2-PE stress occurs at the post-transcriptional level, rather than at the transcriptional level.
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Exposure to microplastics leads to a defective ovarian function and change in cytoskeleton protein expression in rat
Asma Haddadi,Kaouthar Kessabi,Sana Boughammoura,Mariem Ben Rhouma,Rania Mlouka,Mohamed Banni,Imed Messaoudi +6 more
TL;DR: The results uncovered, for the first time, the distribution and accumulation of PS-MPs across rat ovary, revealed a significant alteration in some biomarkers of the ovarian function, and highlighted the possible involvement of MP-induced disturbance of cytoskeleton in these adverse effects.
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Investigation of cellular effects of thymoquinone on glioma cell.
Eray Metin Guler,Eray Metin Guler,Behice Hande Sisman,Abdurrahim Kocyigit,Mustafa Aziz Hatiboglu +4 more
TL;DR: In this paper, the effect of TQ on C6 rat glioma cells was investigated and the results suggest that TQ would be an effective treatment for glioblastoma.
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Enzyme immobilisation on wood-derived cellulose scaffolds via carbohydrate-binding module fusion constructs
Aled D. Roberts,Karl A. P. Payne,Sebastian C. Cosgrove,Viranga Tilakaratna,Itziar Peñafiel,William Finnigan,Nicholas J. Turner,Nigel S. Scrutton +7 more
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References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.