Book ChapterDOI
The Bradford Method for Protein Quantitation
Reads0
Chats0
TLDR
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.Abstract:
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.read more
Citations
More filters
Journal Article
Bcl-xL overexpression and its association with the progress of tongue carcinoma.
TL;DR: The expression of Bcl-xL was significantly higher in tongue carcinoma tissues than in normal tongue tissues and was positively associated with the degree of differentiation and the clinical TNM staging, but negatively correlated with thedegree of malignancy of the tumor.
Journal ArticleDOI
Effect of melilotus extract on lung injury by upregulating the expression of cannabinoid CB2 receptors in septic rats.
Ming-wei Liu,Mei-xian Su,Yun-Hui Wang,Wei Wei,Lan-fang Qin,Xu Liu,Mao-li Tian,Chuan-Yun Qian +7 more
TL;DR: Melilotus extract significantly reduced CLP-induced lung inflammation by up-regulating CB2 expression, and the number of neutrophils, lymphocytes and total cells were significantly lower in the melilotus Extract group than the control group.
Journal ArticleDOI
Astragalus Polysaccharide Inhibits Autophagy and Apoptosis from Peroxide-Induced Injury in C2C12 Myoblasts
Yi Yin,Lu Lu,Dong-Tao Wang,Ying Shi,Ming Wang,Yan-Feng Huang,De-Xiu Chen,Cong Deng,Jie-Bin Chen,Pei-Jia Lv,Yanjing Wang,Chengjie Li,Lian-bo Wei +12 more
TL;DR: APS inhibits the autophagy and apoptosis induced by peroxide injury in C2C12 myoblasts through two independent signaling pathways: the mTOR-independent pathway for the inhibition of autophophagy, and the caspase-3-dependent pathways for the suppression of apoptosis.
Journal ArticleDOI
Immobilization of horseradish peroxidase on polyglycerol-functionalized magnetic Fe3O4/nanodiamond nanocomposites and its application in phenol biodegradation
TL;DR: In this paper, a horseradish peroxidase (HRP) was successfully immobilized on the PG layer of a Fe3O4/nanodiamond nanocomposites (MND) by interaction between functional groups of MND and HRP, where the HRP molecules became tridimensionally connected outside the MND.
Journal ArticleDOI
In vitro construction of artificial blood vessels using spider silk as a supporting matrix.
TL;DR: Spider silk scaffolds seem to provide an optimal and stable basis for vessel constructs that resembles native blood vessels in morphological structure as well as in function and expression of biomarkers.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
Journal ArticleDOI
Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
TL;DR: The Coomassie brilliant blue G assay for proteins described by Bradford (1976) (Anal Biochem72, 248) was reexamined and it was found that the extinction coefficient of the dye-protein complex solution remained constant over the protein concentration range of 08 to 10 μg/ml of solution.
Journal ArticleDOI
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
S.M. Read,D. H. Northcote +1 more
TL;DR: Modifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins.