The Coronavirus Spike Protein Is a Class I Virus Fusion Protein: Structural and Functional Characterization of the Fusion Core Complex
TLDR
Using biological assays, the HR2 peptide was shown to be a potent inhibitor of virus entry into the cell, as well as of cell-cell fusion.Citations
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Journal ArticleDOI
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation.
Daniel Wrapp,Nianshuang Wang,Kizzmekia S. Corbett,Jory A. Goldsmith,Ching-Lin Hsieh,Olubukola M. Abiona,Barney S. Graham,Jason S. McLellan +7 more
TL;DR: The authors show that this protein binds at least 10 times more tightly than the corresponding spike protein of severe acute respiratory syndrome (SARS)–CoV to their common host cell receptor, and test several published SARS-CoV RBD-specific monoclonal antibodies found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs.
Journal ArticleDOI
Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein.
Alexandra C. Walls,Young-Jun Park,M. Alejandra Tortorici,M. Alejandra Tortorici,Abigail Wall,Andrew T. McGuire,Andrew T. McGuire,David Veesler +7 more
TL;DR: It is demonstrating that cross-neutralizing antibodies targeting conserved S epitopes can be elicited upon vaccination, and it is shown that SARS-CoV-2 S uses ACE2 to enter cells and that the receptor-binding domains of Sars- coV- 2 S and SARS S bind with similar affinities to human ACE2, correlating with the efficient spread of SATS among humans.
Journal ArticleDOI
Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus.
Wenhui Li,Michael Moore,Natalya Vasilieva,Jianhua Sui,Swee Kee Wong,Michael A. Berne,Mohan Somasundaran,John L. Sullivan,Katherine Luzuriaga,Thomas C. Greenough,Hyeryun Choe,Michael Farzan +11 more
TL;DR: It is found that a soluble form of ACE2, but not of the related enzyme ACE1, blocked association of the S1 domain with Vero E6 cells, indicating that ACE2 is a functional receptor for SARS-CoV.
Book ChapterDOI
Coronaviruses: An Overview of Their Replication and Pathogenesis
Anthony R. Fehr,Stanley Perlman +1 more
TL;DR: A brief introduction to coronaviruses is provided discussing their replication and pathogenicity, and current prevention and treatment strategies, and the outbreaks of the highly pathogenic Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and the recently identified Middle Eastern Respiratories Syndrome Cor onavirus
Book ChapterDOI
The molecular biology of coronaviruses.
TL;DR: This review summarizes both classical and contemporary discoveries in the study of the molecular biology of these infectious agents, with particular emphasis on the nature and recognition of viral receptors, viral RNA synthesis, and the molecular interactions governing virion assembly.
References
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Journal ArticleDOI
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
H. Schägger,G. von Jagow +1 more
TL;DR: A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described, and the omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing.
Journal ArticleDOI
Calculation of protein extinction coefficients from amino acid sequence data
Stanley C. Gill,P H von Hippel +1 more
TL;DR: In this article, a method for calculating accurate molar extinction coefficients for proteins at 280 nm, simply from knowledge of the amino acid composition, was presented, and the method was calibrated against 18 "normal" globular proteins.
Journal ArticleDOI
Identification of a novel coronavirus in patients with severe acute respiratory syndrome.
Christian Drosten,Stephan Günther,Wolfgang Preiser,Sylvie van der Werf,H. R. Brodt,Stephan Becker,Holger F. Rabenau,Marcus Panning,Larissa Kolesnikova,Ron A. M. Fouchier,Annemarie Berger,Ana-Maria Burguière,Jindrich Cinatl,Markus Eickmann,Nicolas Escriou,Klaus Grywna,Stefanie Kramme,Jean-Claude Manuguerra,Stefanie Müller,Volker Rickerts,Martin Stürmer,Simon Vieth,Hans-Dieter Klenk,Albert D. M. E. Osterhaus,Herbert Schmitz,Hans Wilhelm Doerr +25 more
TL;DR: The novel coronavirus might have a role in causing SARS and was detected in a variety of clinical specimens from patients with SARS but not in controls.
Journal ArticleDOI
A novel coronavirus associated with severe acute respiratory syndrome.
Thomas G. Ksiazek,Dean D. Erdman,Cynthia S. Goldsmith,Zaki,Teresa C. T. Peret,Shannon L. Emery,Suxiang Tong,Urbani C,James A. Comer,Wilina Lim,Pierre E. Rollin,Scott F. Dowell,Ai Ee Ling,Charles D. Humphrey,Wun-Ju Shieh,Jeannette Guarner,Christopher D. Paddock,Paul A. Rota,Barry S. Fields,Joseph L. DeRisi,Jyh-Yuan Yang,Nancy J. Cox,James M. Hughes,James W. LeDuc,William J. Bellini,Larry J. Anderson +25 more
TL;DR: A novel coronavirus is associated with this outbreak of severe acute respiratory syndrome, and the evidence indicates that this virus has an etiologic role in SARS.
Journal ArticleDOI
Coronavirus as a possible cause of severe acute respiratory syndrome
Jsm Peiris,Sik To Lai,Llm Poon,Yi Guan,Lyc Yam,Wilina Lim,John M. Nicholls,Wks Yee,WW Yan,MT Cheung,V. C. C. Cheng,KH Chan,Dnc Tsang,R Yung,Ting Kin Ng,KY Yuen +15 more
TL;DR: Serological and molecular tests specific for the virus permitted a definitive laboratory diagnosis to be made and allowed further investigation to define whether other cofactors play a part in disease progression.
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Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more