Journal ArticleDOI
The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid.
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TLDR
Method has been applied to a study of hydroxyproline distribution in cell particulates, tissue fluids, and purified plant and animal proteins, and significant amounts of hydroXYproline were found in crystalline preparations of pepsin, elastase.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1961-05-01. It has received 3756 citations till now. The article focuses on the topics: Hydroxyproline & Imino acid.read more
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In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel.
Christopher G. Williams,Tae Kyun Kim,Anya Taboas,Athar N. Malik,Paul N. Manson,Jennifer H. Elisseeff +5 more
TL;DR: The ability to encapsulate MSCs to form cartilage-like tissue in vitro in a photopolymerizing hydrogel may be useful for minimally invasive implantation, MSC differentiation, and engineering of composite tissue structures with multiple cellular phenotypes.
Journal ArticleDOI
Transient gene transfer and expression of Smad7 prevents bleomycin-induced lung fibrosis in mice
Atsuhito Nakao,Makiko Fujii,Ryutaro Matsumura,Kotaro Kumano,Yasushi Saito,Kohei Miyazono,Itsuo Iwamoto +6 more
TL;DR: Exogenous Smad7 introduced by a recombinant human type 5 adenovirus vector prevented bleomycin-induced lung fibrosis in mice, suggesting that Smad 7 may have applicability in the treatment of pulmonary fibrosis.
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Topical Estrogen Accelerates Cutaneous Wound Healing in Aged Humans Associated with an Altered Inflammatory Response
Gillian S. Ashcroft,Gillian S. Ashcroft,Teresa Greenwell-Wild,Michael A Horan,Sharon M. Wahl,Mark William James Ferguson +5 more
TL;DR: Estrogen treatment increased the extent of wound healing in both males and females with a decrease in wound size at day 7, increased collagen levels at both days 7 and 80, and increased day 7 fibronectin levels.
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In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica
TL;DR: Results indicate that asiaticoside exhibits significant wound healing activity in normal as well as delayed healing models and is the main active constituent of Centella asiaticsa.
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Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds
TL;DR: Six month repair was qualitatively better for cell-PGA allografts than for PGA alone, with respect to: 1) surface smoothness, 2) columnar alignment of chondrocytes, 3) spatially uniform GAG distribution, 4) reconstitution of the subchondral plate, and 5) bonding of the repair tissue to the underlying bone.
References
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Journal ArticleDOI
The determination of hydroxyproline.
Robert E. Neuman,Milan A. Logan +1 more
TL;DR: A short and simple calorimetric method is here described that is applicable to the determination of hydroxyproline in hydrolysates of 40 to 100 y of collagen with a reproducibility of f2 per cent and an accuracy of f1 per cent as judged by recovery of hydroXYproline from elastin hydrolysate and from an amino acid mixture simulating collagen.
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[Microdetermination of hydroxyproline with chloramine-T and p-dimethylaminobenzaldehyde].
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Growth and sexual differentiation of hydra in mass culture
W. F. Loomis,H. M. Lenhoff +1 more
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Appendix—Notes on a modification of the Neuman & Logan method for the determination of the hydroxyproline
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Formation of collagen hydroxyproline in vitro
N. M. Green,D. A. Lowther +1 more
TL;DR: Stetten (1949) suggested that free hydroxyproline was not an intermediate in collagen biosynthesis and that hydroxylation of the proline residues occurred at the protein or peptide stage.